The CD27L and CTP1L endolysins targeting Clostridia contain a built-in trigger and release factor.

Dunne M, Mertens HD, Garefalaki V, Jeffries CM, Thompson A, Lemke EA, Svergun DI, Mayer MJ, Narbad A, Meijers R, PLoS Pathog 10(7):e1004228 (2014) Europe PMC

SASDAM5 – Clostridium difficile bacteriophage 27 endolysin dimer C238R mutant, CD27L-C238R

Clostridium difficile bacteriophage 27 endolysin C238R mutant
MWI(0) 64 kDa
MWexpected 64 kDa
VPorod 92 nm3
log I(s) 3.66×103 3.66×102 3.66×101 3.66×100
Clostridium difficile bacteriophage 27 endolysin C238R mutant small angle scattering data  s, nm-1
ln I(s)
Clostridium difficile bacteriophage 27 endolysin C238R mutant Guinier plot ln 3.66×103 Rg: 4.2 nm 0 (4.2 nm)-2 s2
(sRg)2I(s)/I(0)
Clostridium difficile bacteriophage 27 endolysin C238R mutant Kratky plot 1.104 0 √3 sRg
p(r)
Clostridium difficile bacteriophage 27 endolysin C238R mutant pair distance distribution function Rg: 4.3 nm 0 Dmax: 14.7 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Clostridium difficile bacteriophage 27 endolysin C238R mutant DAMMIF model
log I(s)
 s, nm-1
Clostridium difficile bacteriophage 27 endolysin C238R mutant CORAL model
log I(s)
 s, nm-1
Clostridium difficile bacteriophage 27 endolysin C238R mutant EOM/RANCH model
Synchrotron SAXS data from solutions of Clostridium difficile bacteriophage 27 endolysin dimer C238R mutant, CD27L-C238R in 20 mM HEPES 500 mM NaCl, pH 7.5 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.12 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1 and 8.5 mg/ml were measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Clostridium difficile bacteriophage 27 endolysin C238R mutant (CD27L(C238R))
Mol. type   Protein
Organism   Clostridioides difficile
Olig. state   Dimer
Mon. MW   32.1 kDa
Sequence   FASTA