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Synchrotron SAXS data from solutions of complement C3* in 20 mM MES pH 6.0, 200 mM NaCl, pH 6 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.25 mg/ml was measured at 8°C. 40 successive 0.095 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
CAUTION! The low-angle SAXS intensities encompassing the entire Guinier region - and beyond to higher angle - (0.03 < s < 0.34 nm-1) have been removed when assessing the model fit to the data. Consequently, the model fit and particle sizing cannot be validated (e.g., Rg(model) = 4.8 nm; Rg(experiment) = 5.5 nm; Dmax model = 14.6 nm; Dmax(experiment) = 21.8 nm, etc).
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s, nm-1
