Structural and Functional Characterization of Rv0792c from Mycobacterium tuberculosis: Identifying Small Molecule Inhibitor against HutC Protein.

Chauhan NK, Anand A, Sharma A, Dhiman K, Gosain TP, Singh P, Singh P, Khan E, Chattopadhyay G, Kumar A, Sharma D, Ashish, Sharma TK, Singh R, Microbiol Spectr :e0197322 (2022) Europe PMC

SASDLS8 – Complex of Mtb GntR and Aptamer 1 [Rv0792c and Rv0792c_1]

Complex of Rv0792c and Rv0792c_1
MWexperimental 86 kDa
MWexpected 74 kDa
log I(s) 1.40×103 1.40×102 1.40×101 1.40×100
Complex of Rv0792c and Rv0792c_1 small angle scattering data  s, nm-1
ln I(s)
Complex of Rv0792c and Rv0792c_1 Guinier plot ln 1.40×103 Rg: 3.6 nm 0 (3.6 nm)-2 s2
(sRg)2I(s)/I(0)
Complex of Rv0792c and Rv0792c_1 Kratky plot 1.104 0 √3 sRg
p(r)
Complex of Rv0792c and Rv0792c_1 pair distance distribution function Rg: 3.5 nm 0 Dmax: 12 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Complex of Rv0792c and Rv0792c_1 OTHER model
log I(s)
 s, nm-1
Complex of Rv0792c and Rv0792c_1 DAMFILT model
Complex of Rv0792c and Rv0792c_1 OTHER model
Complex of Rv0792c and Rv0792c_1 DAMMIF model
SAXS data from solutions of Complex of Mtb GntR and Aptamer 1 [Rv0792c and Rv0792c_1] in 25 mM HEPES Buffer; 400 mM NaCl, pH 7.2 were collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) using a Mythen 1K detector at a sample-detector distance of 0.3 m and at a wavelength of λ = 0.154141 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 10°C. Three successive 3600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Complex of Rv0792c and Rv0792c_1 (complex1)
Mol. type   Other
Olig. state   Monomer
Mon. MW   74 kDa
Chemical formula