Isolation and oligomeric composition of cytochrome c nitrite reductase from the haloalkaliphilic bacterium Thioalkalivibrio nitratireducens.

Tikhonova TV, Slutskaya ES, Filimonenkov AA, Boyko KM, Kleimenov SY, Konarev PV, Polyakov KM, Svergun DI, Trofimov AA, Khomenkov VG, Zvyagilskaya RA, Popov VO, Biochemistry (Mosc) 73(2):164-70 (2008) Europe PMC

SASDLW5 – Cytochrome c nitrite reductase from Thioalkalivibrio nitratireducens

Cytochrome c-552
MWI(0) 370 kDa
MWexpected 356 kDa
log I(s) 6.32×104 6.32×103 6.32×102 6.32×101
Cytochrome c-552 small angle scattering data  s, nm-1
ln I(s)
Cytochrome c-552 Guinier plot ln 6.33×104 Rg: 4.8 nm 0 (4.8 nm)-2 s2
(sRg)2I(s)/I(0)
Cytochrome c-552 Kratky plot 1.104 0 √3 sRg

Data validation

Fits and models

log I(s)
 s, nm-1
Cytochrome c-552 PDB (PROTEIN DATA BANK) model
Cytochrome c-552 PDB (PROTEIN DATA BANK) model
Synchrotron SAXS data from solutions of cytochrome c nitrite reductase in Tris-borate buffer, pH 8.5 were collected on the EMBL X33 beam line at DORIS III (DESY, Hamburg, Germany) using a MAR 345 Image Plate detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.50 mg/ml was measured at 20°C. One 120 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Tags: X33
Cytochrome c-552 (Nitrite reductase)
Mol. type   Protein
Organism   Thioalkalivibrio nitratireducens (strain DSM 14787 / UNIQEM 213 / ALEN2)
Olig. state   Hexamer
Mon. MW   59.4 kDa
 
UniProt   L0DSL2 (29-553)
Sequence   FASTA
 
PDB ID   2OT4
 
PDB ID   2OT4