TIA-1 RRM23 binding and recognition of target oligonucleotides.

Waris S, García-Mauriño SM, Sivakumaran A, Beckham SA, Loughlin FE, Gorospe M, Díaz-Moreno I, Wilce MCJ, Wilce JA, Nucleic Acids Res 45(8):4944-4957 (2017) Europe PMC

SASDBQ6 – Nucleolysin TIA-1 isoform p40 in complex with TTTTTACTCC DNA

Nucleolysin TIA-1 isoform p40
DNA (TTTTTACTCC)
MWexperimental 23 kDa
MWexpected 22 kDa
VPorod 32 nm3
log I(s) 4.30×10-2 4.30×10-3 4.30×10-4 4.30×10-5
Nucleolysin TIA-1 isoform p40 DNA (TTTTTACTCC) small angle scattering data  s, nm-1
ln I(s)
Nucleolysin TIA-1 isoform p40 DNA (TTTTTACTCC) Guinier plot ln 4.30×10-2 Rg: 2.1 nm 0 (2.1 nm)-2 s2
(sRg)2I(s)/I(0)
Nucleolysin TIA-1 isoform p40 DNA (TTTTTACTCC) Kratky plot 1.104 0 √3 sRg
p(r)
Nucleolysin TIA-1 isoform p40 DNA (TTTTTACTCC) pair distance distribution function Rg: 2.2 nm 0 Dmax: 7.1 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Nucleolysin TIA-1 isoform p40 in complex with DNA (TTTTTACTCC) in 20 mM HEPES, 100mM NaCl, 3% v/v glycerol, pH 7 were collected on the SAXS/WAXS beam line at the Australian Synchrotron (Melbourne, Australia) using a Pilatus 1M detector at a sample-detector distance of 1.6 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). The data were collected as 30 successive 1 second frames and were normalized to the intensity of the transmitted beam and radially averaged. The radially-averaged scattering of the solvent-blank was subtracted to produce the results displayed in this entry that shows the scattering profile from a sample at 2.5 mg/ml maintained at 15°C during the SAXS measurements.

TIA-1 RRM23 + TC-rich DNA

Nucleolysin TIA-1 isoform p40 (TIA-1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   21.3 kDa
 
UniProt   P31483-2 (93-274)
Sequence   FASTA
 
DNA (TTTTTACTCC)
Mol. type   DNA
Olig. state   Monomer
Mon. MW   0.8 kDa
Sequence   FASTA