Search

  
Advanced search  

3 hits found for DNA 22mer

SASDS97 – ...DNA in the presence of RNA initiation factors

DNA polymerase alpha subunit BDNA polymerase alpha catalytic subunitDNA primase large subunitDNA primase small subunit22mer DNA template experimental SAS data
DNA polymerase alpha subunit B DNA polymerase alpha catalytic subunit DNA primase large subunit DNA primase small subunit 22mer DNA template Kratky plot
Sample: DNA polymerase alpha subunit B monomer, 49 kDa Homo sapiens protein
DNA polymerase alpha catalytic subunit monomer, 23 kDa Homo sapiens protein
DNA primase large subunit monomer, 59 kDa Homo sapiens protein
DNA primase small subunit monomer, 50 kDa Homo sapiens protein
22mer DNA template monomer, 7 kDa DNA
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM MnCl2, 1 mM TCEP, 1 mM 5'-guanylylmethylenediphosphonate (GMPCPP), pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 4
...DNA polymerase α-primase Journal of Molecular Biology :168330 (2023)
Cordoba J, Mullins E, Salay L, Eichman B, Chazin W
RgGuinier 4.6 nm
Dmax 14.2 nm
VolumePorod 310 nm3

SASDAQ7 – ...DNA complex

Pre-B-cell leukemia transcription factor 1Homeobox protein PKNOX1DNA 22mer experimental SAS data
DAMMIF model
Sample: Pre-B-cell leukemia transcription factor 1 monomer, 34 kDa Homo sapiens protein
Homeobox protein PKNOX1 monomer, 38 kDa Homo sapiens protein
DNA 22mer monomer, 12 kDa DNA
Buffer: 20 mM Tris-HCl 150 mM NaCl 5% glycerol 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 6
...DNA binding. FEBS J 283(16):3134-54 (2016)
Mathiasen L, Valentini E, Boivin S, Cattaneo A, Blasi F, Svergun DI, Bruckmann C
RgGuinier 4.8 nm
Dmax 16.3 nm
VolumePorod 173 nm3

SASDAR7 – DNA 22mer

DNA 22mer experimental SAS data
DAMMIF model
Sample: DNA 22mer monomer, 12 kDa DNA
Buffer: 20 mM Tris-HCl 150 mM NaCl 5% glycerol 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 6
...DNA binding. FEBS J 283(16):3134-54 (2016)
Mathiasen L, Valentini E, Boivin S, Cattaneo A, Blasi F, Svergun DI, Bruckmann C
RgGuinier 2.2 nm
Dmax 7.5 nm
VolumePorod 19 nm3