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9 hits found for Diguanylate cyclase

SASDUS3 – ...diguanylate cyclase fusion from Acidimicrobiaceae bacterium

GGDEF domain-containing protein experimental SAS data
DAMMIF model
Sample: GGDEF domain-containing protein dimer, 132 kDa Acidimicrobiaceae bacterium protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 6.8
Experiment: SAXS data collected at BM29, ESRF on 2022 Jan 21
Photocobilins integrate B(12) and bilin photochemistry for enzyme control. Nat Commun 15(1):2740 (2024)
Zhang S, Jeffreys LN, Poddar H, Yu Y, Liu C, Patel K, Johannissen LO, Zhu L, Cliff MJ, Yan C, Schirò G, Weik M, Sakuma M, Levy CW, Leys D, Heyes DJ, Scrutton NS
RgGuinier 4.1 nm
Dmax 14.9 nm
VolumePorod 194 nm3

SASDUT3 – ...diguanylate cyclase fusion from Acidimicrobiaceae bacterium

GGDEF domain-containing protein experimental SAS data
DAMMIF model
Sample: GGDEF domain-containing protein dimer, 132 kDa Acidimicrobiaceae bacterium protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 6.8
Experiment: SAXS data collected at BM29, ESRF on 2022 Jan 21
Photocobilins integrate B(12) and bilin photochemistry for enzyme control. Nat Commun 15(1):2740 (2024)
Zhang S, Jeffreys LN, Poddar H, Yu Y, Liu C, Patel K, Johannissen LO, Zhu L, Cliff MJ, Yan C, Schirò G, Weik M, Sakuma M, Levy CW, Leys D, Heyes DJ, Scrutton NS
RgGuinier 5.0 nm
Dmax 18.5 nm
VolumePorod 170 nm3

SASDSA5 – ...diguanylate cyclase

Diguanylate cyclase experimental SAS data
Diguanylate cyclase Kratky plot
Sample: Diguanylate cyclase dimer, 67 kDa Aquella oligotrophica protein
Buffer: 10 mM Tris, 500 mM NaCl, 2 mM MgCl2, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 May 4
...diguanylate cyclases Science Advances 9(31) (2023)
Vide U, Kasapović D, Fuchs M, Heimböck M, Totaro M, Zenzmaier E, Winkler A
RgGuinier 3.4 nm
Dmax 13.6 nm
VolumePorod 102 nm3

SASDSB5 – ...diguanylate cyclase

Diguanylate cyclase experimental SAS data
Diguanylate cyclase Kratky plot
Sample: Diguanylate cyclase dimer, 67 kDa Aquella oligotrophica protein
Buffer: 10 mM Tris, 500 mM NaCl, 2 mM MgCl2, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 May 4
...diguanylate cyclases Science Advances 9(31) (2023)
Vide U, Kasapović D, Fuchs M, Heimböck M, Totaro M, Zenzmaier E, Winkler A
RgGuinier 3.4 nm
Dmax 15.7 nm
VolumePorod 104 nm3

SASDSC5 – ...diguanylate cyclase

Sensor domain-containing diguanylate cyclase experimental SAS data
GASBOR model
Sample: ...diguanylate cyclase dimer, 72 kDa Methylotenera sp. protein
Buffer: 10 mM Tris, 50 mM NaCl, 2 mM MgCl2, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 May 4
...diguanylate cyclases Science Advances 9(31) (2023)
Vide U, Kasapović D, Fuchs M, Heimböck M, Totaro M, Zenzmaier E, Winkler A
RgGuinier 2.9 nm
Dmax 11.1 nm
VolumePorod 115 nm3

SASDSD5 – ...diguanylate cyclase

Sensor domain-containing diguanylate cyclase experimental SAS data
Sensor domain-containing diguanylate cyclase Kratky plot
Sample: ...diguanylate cyclase dimer, 72 kDa Methylotenera sp. protein
Buffer: 10 mM Tris, 50 mM NaCl, 2 mM MgCl2, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 May 4
...diguanylate cyclases Science Advances 9(31) (2023)
Vide U, Kasapović D, Fuchs M, Heimböck M, Totaro M, Zenzmaier E, Winkler A
RgGuinier 3.5 nm
Dmax 13.8 nm
VolumePorod 123 nm3

SASDC96 – ...Diguanylate cyclase with PAS/PAC sensor (Maqu_2914) from Marinobacter aquaeolei, Northeast Structural Genomics Consortium Target MqR66C

Diguanylate cyclase with PAS/PAC sensor experimental SAS data
DAMFILT model
Sample: Diguanylate cyclase with PAS/PAC sensor dimer, 27 kDa Marinobacter hydrocarbonoclasticus protein
Buffer: 5 mM DTT 100 mM NaCl 10 mM Tris-HCl 0.02 % NaN3, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2010 Feb 12
Small angle X-ray scattering as a complementary tool for high-throughput structural studies. Biopolymers 95(8):517-30 (2011)
Grant TD, Luft JR, Wolfley JR, Tsuruta H, Martel A, Montelione GT, Snell EH
RgGuinier 2.0 nm
Dmax 6.7 nm
VolumePorod 41 nm3

SASDCC6 – Sensory box domain of the sensory-box/GGDEF protein SO_1695 from Shewanella oneidensis, Northeast Structural Genomics Consortium Target SoR288B

Diguanylate cyclase with PAS sensory domain experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Diguanylate cyclase with PAS sensory domain dimer, 29 kDa Shewanella oneidensis protein
Buffer: 5 mM DTT 100 mM NaCl 10 mM Tris-HCl 0.02 % NaN3, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2010 Feb 12
Small angle X-ray scattering as a complementary tool for high-throughput structural studies. Biopolymers 95(8):517-30 (2011)
Grant TD, Luft JR, Wolfley JR, Tsuruta H, Martel A, Montelione GT, Snell EH
RgGuinier 2.0 nm
Dmax 6.4 nm
VolumePorod 48 nm3

SASDCH6 – ...diguanylate cyclase complexed with c-di-GMP - Northeast Structural Genomics Consortium Target MqR89a

Diguanylate cyclase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Diguanylate cyclase monomer, 20 kDa Marinobacter hydrocarbonoclasticus protein
Buffer: 5 mM DTT 100 mM NaCl 10 mM Tris-HCl 0.02 % NaN3, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2010 Feb 12
Small angle X-ray scattering as a complementary tool for high-throughput structural studies. Biopolymers 95(8):517-30 (2011)
Grant TD, Luft JR, Wolfley JR, Tsuruta H, Martel A, Montelione GT, Snell EH
RgGuinier 1.9 nm
Dmax 6.6 nm
VolumePorod 32 nm3