Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research.

Flayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C, Structure 26(2):345-355.e5 (2018) Europe PMC

SASDCZ6 – DtpA SapNP (SapA:POPS) (SEC-SAXS)

saposin-a
1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine
Dipeptide and tripeptide permease A
MWexperimental 139 kDa
MWexpected 107 kDa
VPorod 288 nm3
log I(s) 3.31×103 3.31×102 3.31×101 3.31×100
saposin-a 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine Dipeptide and tripeptide permease A small angle scattering data  s, nm-1
ln I(s)
saposin-a 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine Dipeptide and tripeptide permease A Guinier plot ln 3.31×103 Rg: 4 nm 0 (4 nm)-2 s2
(sRg)2I(s)/I(0)
saposin-a 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine Dipeptide and tripeptide permease A Kratky plot 1.104 0 3 sRg
p(r)
saposin-a 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine Dipeptide and tripeptide permease A pair distance distribution function Rg: 4 nm 0 Dmax: 13.5 nm

Data validation


Fits and models


log I(s)
 s, nm-1
saposin-a 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine Dipeptide and tripeptide permease A DAMMIF model

Synchrotron SAXS data from solutions of DtpA SapNP (SapA:POPS) (SEC-SAXS) in PBS, pH 7.4 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). at 10°C. 3600 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Concentration = UNKNOWN

saposin-a (sapA)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   9.1 kDa
 
UniProt   P07602 (60-140)
Sequence   FASTA
 
1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS)
Mol. type   Other
Organism   synthetic construct
Olig. state   Other
Mon. MW   0.8 kDa
Dipeptide and tripeptide permease A (DtpA)
Mol. type   Protein
Organism   Escherichia coli
Olig. state   Monomer
Mon. MW   51.1 kDa
 
UniProt   P77304 (17-487)
Sequence   FASTA