Crystal structure of an extracellular superoxide dismutase from Onchocerca volvulus and implications for parasite-specific drug development

Moustafa A, Perbandt M, Liebau E, Betzel C, Falke S, Acta Crystallographica Section F Structural Biology Communications 78(6) (2022) DOI

SASDPF2 – Structure of an extracellular superoxide dismutase from Onchocerca volvulus

Extracellular superoxide dismutase [Cu-Zn]
MWI(0) 38 kDa
MWexpected 34 kDa
VPorod 55 nm3
log I(s) 6.92×103 6.92×102 6.92×101 6.92×100
Extracellular superoxide dismutase [Cu-Zn] small angle scattering data  s, nm-1
ln I(s)
Extracellular superoxide dismutase [Cu-Zn] Guinier plot ln 6.92×103 Rg: 2.6 nm 0 (2.6 nm)-2 s2
(sRg)2I(s)/I(0)
Extracellular superoxide dismutase [Cu-Zn] Kratky plot 1.104 0 √3 sRg
p(r)
Extracellular superoxide dismutase [Cu-Zn] pair distance distribution function Rg: 2.6 nm 0 Dmax: 9 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Extracellular superoxide dismutase [Cu-Zn] GASBOR model
log I(s)
 s, nm-1
Extracellular superoxide dismutase [Cu-Zn] PDB (PROTEIN DATA BANK) model
log I(s)
 s, nm-1
Extracellular superoxide dismutase [Cu-Zn] SREFLEX model
Synchrotron SAXS data from solutions of an extracellular superoxide dismutase from Onchocerca volvulus in 50 mM Tris-HCl, 200 mM NaCl, 2 mM DTT, pH 8 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.5 and 7.5 mg/ml were measured at 20°C. 20 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Extracellular superoxide dismutase [Cu-Zn]
Mol. type   Protein
Organism   Onchocerca volvulus
Olig. state   Dimer
Mon. MW   17.0 kDa
 
UniProt   Q07449 (44-201)
Sequence   FASTA
 
PDB ID   5IN2