The central portion of factor H (modules 10-15) is compact and contains a structurally deviant CCP module.

Schmidt CQ, Herbert AP, Mertens HD, Guariento M, Soares DC, Uhrin D, Rowe AJ, Svergun DI, Barlow PN, J Mol Biol 395(1):105-22 (2010) Europe PMC

SASDAZ4 – fH1114

Factor H CCP modules 11 to 14
MWI(0) 35 kDa
MWexpected 28 kDa
VPorod 38 nm3
log I(s) 3.80×10-1 3.80×10-2 3.80×10-3 3.80×10-4
Factor H CCP modules 11 to 14 small angle scattering data  s, nm-1
ln I(s)
Factor H CCP modules 11 to 14 Guinier plot ln 3.80×10-1 Rg: 3 nm 0 (3 nm)-2 s2
(sRg)2I(s)/I(0)
Factor H CCP modules 11 to 14 Kratky plot 1.104 0 √3 sRg
p(r)
Factor H CCP modules 11 to 14 pair distance distribution function Rg: 3.1 nm 0 Dmax: 10.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Factor H CCP modules 11 to 14 DAMMIF model
log I(s)
 s, nm-1
Factor H CCP modules 11 to 14 GASBOR model
log I(s)
 s, nm-1
Factor H CCP modules 11 to 14 BUNCH model
Synchrotron SAXS data from solutions of fH1114 in 50 mM Potassium Phosphate, pH 7.4 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a MAR 345 Image Plate detector (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.7 and 1.6 mg/ml were measured at 10°C. One 120 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Wavelength = UNKNOWN. Sample detector distance = UNKNOWN

Tags: X33
Factor H CCP modules 11 to 14 (fH1114)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   27.6 kDa
Sequence   FASTA