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6 hits found for Genome polyprotein

SASDTR2 – ...polyprotein fragment at pH 5.4

Genome polyprotein experimental SAS data
ALPHAFOLD model
Sample: Genome polyprotein monomer, 13 kDa Turkey avisivirus protein
Buffer: 20 mM citrate pH 5.4, 150 mM NaCl, 2 mM TCEP, 1% glycerol, pH: 5.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 May 2
Solution structure and functional characterization of an avian picornavirus 2AH/NC protein
Anna Wehlin
RgGuinier 1.7 nm
Dmax 7.2 nm
VolumePorod 29 nm3

SASDTS2 – ...polyprotein fragment at pH 6.5

Genome polyprotein experimental SAS data
ALPHAFOLD model
Sample: Genome polyprotein monomer, 13 kDa Turkey avisivirus protein
Buffer: 20mM Citrate buffer pH 6.5, 150mM NaCl, 2mM TCEP, 1% glycerol, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 May 15
Solution structure and functional characterization of an avian picornavirus 2AH/NC protein
Anna Wehlin
RgGuinier 1.6 nm
Dmax 6.0 nm
VolumePorod 27 nm3

SASDTT2 – ...polyprotein fragment at pH 7.4

Genome polyprotein experimental SAS data
ALPHAFOLD model
Sample: Genome polyprotein monomer, 13 kDa Turkey avisivirus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM TECP, 1% Glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 May 2
Solution structure and functional characterization of an avian picornavirus 2AH/NC protein
Anna Wehlin
RgGuinier 1.6 nm
Dmax 4.0 nm
VolumePorod 24 nm3

SASDWQ5 – 2A protein from Theiler's murine encephalomyelitis virus (TMEV)

Genome polyprotein experimental SAS data
CORAL model
Sample: Genome polyprotein monomer, 17 kDa Theiler's murine encephalomyelitis … protein
Buffer: 20 mM HEPES pH 7.4, 1 M KCl, 2% v/v glycerol, 1 mM DTT, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 26
A new protein-dependent riboswitch activates ribosomal frameshifting (2025)
Betts J, Jeffries C, Passchier T, Kung H, Graham S, Abdelhamid M, Howard J, Craggs T, Graham S, Brierley I, Leake M, Quinn S, Hill C
RgGuinier 1.9 nm
Dmax 7.2 nm
VolumePorod 30 nm3

SASDWS5 – 2A protein from Theiler's murine encephalomyelitis virus (TMEV) bound to stimulatory element RNA

Genome polyproteinStimulatory element RNA experimental SAS data
CORAL model
Sample: Genome polyprotein monomer, 17 kDa Theiler's murine encephalomyelitis … protein
Stimulatory element RNA monomer, 12 kDa synthetic RNA RNA
Buffer: 20 mM HEPES pH 7.4, 400 mM KCl 2% v/v glycerol, 1 mM DTT, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 26
A new protein-dependent riboswitch activates ribosomal frameshifting (2025)
Betts J, Jeffries C, Passchier T, Kung H, Graham S, Abdelhamid M, Howard J, Craggs T, Graham S, Brierley I, Leake M, Quinn S, Hill C
RgGuinier 2.2 nm
Dmax 7.5 nm
VolumePorod 46 nm3

SASDML9 – Structure of bifunctional protease-helicase NS3/4A domain assembly in solution

Genome polyprotein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Genome polyprotein dimer, 137 kDa Hepatitis C virus … protein
Buffer: 25 mM Tris, 1 M NaCl, 10% glycerol, 1 mM TCEP, 0.1% β-octyl glucoside, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Dec 12
A macrocyclic HCV NS3/4A protease inhibitor interacts with protease and helicase residues in the complex with its full-length target Proceedings of the National Academy of Sciences 108(52):21052-21056 (2011)
Schiering N, D'Arcy A, Villard F, Simic O, Kamke M, Monnet G, Hassiepen U, Svergun D, Pulfer R, Eder J, Raman P, Bodendorf U
RgGuinier 3.9 nm