HBV G-quad

Trushar Patel.

SASDKP6 – G-quadruplex DNA from Hepatitis B virus, wild type (HBV G4 WT)

HBV G4 WT
MWexperimental 7 kDa
MWexpected 7 kDa
VPorod 18 nm3
log I(s) 3.52×10-3 3.52×10-4 3.52×10-5 3.52×10-6
HBV G4 WT small angle scattering data  s, nm-1
ln I(s)
HBV G4 WT Guinier plot ln 3.53×10-3 Rg: 1.8 nm 0 (1.8 nm)-2 s2
(sRg)2I(s)/I(0)
HBV G4 WT Kratky plot 1.104 0 √3 sRg
p(r)
HBV G4 WT pair distance distribution function Rg: 1.7 nm 0 Dmax: 4.7 nm

Data validation

Fits and models

log I(s)
 s, nm-1
HBV G4 WT DAMMIN model
log I(s)
 s, nm-1
HBV G4 WT DAMMIN model
log I(s)
 s, nm-1
HBV G4 WT DAMMIN model
Synchrotron SAXS data from solutions of G-quadruplex DNA from Hepatitis B virus (wild type) in 20 mM HEPES, 100 mM KCl, 1 mM EDTA, pH 7.5 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Pilatus 2M detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Concentration: UNKNOWN. CAUTION: Dmax appears severely underestimated.

HBV G4 WT (HBV G4 WT)
Mol. type   DNA
Organism   Hepatitis B virus
Olig. state   Monomer
Mon. MW   7.4 kDa
Sequence   FASTA