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2 hits found for HOTag-(PA)25-Ubiquitin

SASDRC7 – HOTag6 tetramerization domain followed by a (Pro-Ala)10 linker fused to human monoubiquitin

HOTag-(PA)25-Ubiquitin experimental SAS data
HOTag-(PA)25-Ubiquitin Kratky plot
Sample: HOTag-(PA)25-Ubiquitin tetramer, 68 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
Galagedera S, Dao T, Enos S, Chaudhuri A, Schmit J, Castañeda C
RgGuinier 5.1 nm
Dmax 18.4 nm
VolumePorod 106 nm3

SASDRK8 – HOTag6 tetramerization domain followed by a (Pro-Ala)25 linker fused to human monoubiquitin

HOTag-(PA)25-Ubiquitin experimental SAS data
HOTag-(PA)25-Ubiquitin Kratky plot
Sample: HOTag-(PA)25-Ubiquitin tetramer, 68 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 22
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
Galagedera S, Dao T, Enos S, Chaudhuri A, Schmit J, Castañeda C
RgGuinier 6.3 nm
Dmax 24.9 nm
VolumePorod 180 nm3