The plant nucleoplasmin AtFKBP43 needs its extended arms for histone interaction.

Singh AK, Saharan K, Baral S, Vasudevan D, Biochim Biophys Acta Gene Regul Mech 1865(7):194872 (2022) Europe PMC

SASDNR7 – Peptidyl-prolyl cis-trans isomerase AtFKBP43 (1-136) -H2A/H2B histone oligomer complex

Peptidyl-prolyl cis-trans isomerase FKBP43
Histone H2A type 1-B/E
Histone H2B type 1-C/E/F/G/I
MWexperimental 107 kDa
MWexpected 109 kDa
VPorod 194 nm3
log I(s) 9.42×101 9.42×100 9.42×10-1 9.42×10-2
Peptidyl-prolyl cis-trans isomerase FKBP43 Histone H2A type 1-B/E Histone H2B type 1-C/E/F/G/I small angle scattering data  s, nm-1
ln I(s)
Peptidyl-prolyl cis-trans isomerase FKBP43 Histone H2A type 1-B/E Histone H2B type 1-C/E/F/G/I Guinier plot ln 9.42×101 Rg: 4.1 nm 0 (4.1 nm)-2 s2
(sRg)2I(s)/I(0)
Peptidyl-prolyl cis-trans isomerase FKBP43 Histone H2A type 1-B/E Histone H2B type 1-C/E/F/G/I Kratky plot 1.104 0 √3 sRg
p(r)
Peptidyl-prolyl cis-trans isomerase FKBP43 Histone H2A type 1-B/E Histone H2B type 1-C/E/F/G/I pair distance distribution function Rg: 4.2 nm 0 Dmax: 13.9 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Peptidyl-prolyl cis-trans isomerase FKBP43 Histone H2A type 1-B/E Histone H2B type 1-C/E/F/G/I MULTIFOXS model
log I(s)
 s, nm-1
Peptidyl-prolyl cis-trans isomerase FKBP43 Histone H2A type 1-B/E Histone H2B type 1-C/E/F/G/I DAMMIF model
Peptidyl-prolyl cis-trans isomerase FKBP43 Histone H2A type 1-B/E Histone H2B type 1-C/E/F/G/I DAMFILT model
Synchrotron SAXS data from solutions of the AtFKBP43 (1-136) -H2A/H2B histone oligomer complex in 20 mM Tris, 300 mM NaCl, 1 mM β-mercaptoethanol, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09794 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.50 mg/ml was measured at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The bead models displayed in this entry show an individual DAMMIF model shape reconstruction and associated fit, in addition to the volume/bead occupancy-corrected averaged representation of the protein calculated from the spatial alignment of an individual model cohort consisting of several individual bead models (DAMFILT).

Peptidyl-prolyl cis-trans isomerase FKBP43 (FKBP43)
Mol. type   Protein
Organism   Arabidopsis thaliana
Olig. state   Pentamer
Mon. MW   16.1 kDa
 
UniProt   F4J9Q6 (1-136)
Sequence   FASTA
 
Histone H2A type 1-B/E (Human H2A)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   14.1 kDa
 
UniProt   P04908 (1-130)
Sequence   FASTA
 
Histone H2B type 1-C/E/F/G/I (Human H2B)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   13.9 kDa
 
UniProt   P62807 (1-126)
Sequence   FASTA