| MWexperimental | 78 | kDa |
| MWexpected | 92 | kDa |
| VPorod | 97 | nm3 |
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log I(s)
1.32×10-1
1.32×10-2
1.32×10-3
1.32×10-4
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s, nm-1
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Isotype Switching Converts Anti-CD40 Antagonism to Agonism to Elicit Potent Antitumor Activity
Yu X, Chan H, Fisher H, Penfold C, Kim J, Inzhelevskaya T, Mockridge C, French R, Duriez P, Douglas L, English V, Verbeek J, White A, Tews I, Glennie M, Cragg M, Cancer Cell (2020) DOISASDHN8 – Human tumor necrosis factor receptor superfamily member 5 (CD40) extracellular domain in complex with human immunoglobulin gamma 1 (IgG1) 341G2 F(ab)
Tumor necrosis factor receptor superfamily member 5Human 341G2 F(ab)
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Fits and models
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Synchrotron SAXS data from solutions of the extracellular domain of CD40 in complex with IgG1 341G2 F(ab) in phosphate-buffered saline, pH 7 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Pilatus 2M detector at a sample-detector distance of 4.0 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 45 μl sample at 7.7 mg/ml was injected at a 0.07 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 25°C. Three successive 620 second frames were collected through the SEC elution. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted from data frames measured from the sample collected through the SEC-elution peak.
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