| MWexperimental | 34 | kDa |
| MWexpected | 34 | kDa |
| VPorod | 44 | nm3 |
|
log I(s)
1.36×102
1.36×101
1.36×100
1.36×10-1
|
s, nm-1
|
Fine-tuning of intrinsic N-Oct-3 POU domain allostery by regulatory DNA targets
Alazard R, Mourey L, Ebel C, Konarev P, Petoukhov M, Svergun D, Erard M, Nucleic Acids Research 35(13):4420-4432 (2007) DOISASDL37 – N-Oct-3 POU transcription factor domain in complex with human DR-alpha DNA
POU domain, class 3, transcription factor 2Human DR-alpha DNA
|
|
|
Fits and models
|
|
|
Synchrotron SAXS
data from solutions of
N-Oct-3 POU transcription factor domain in complex with human DR-alpha DNA
in
50 mM Tris, 0.4 M NaCl, 2% glycerol, 2 mM DTT, pH 7.5
were collected
on the
EMBL X33 beam line
at the DORIS III, DESY storage ring
(Hamburg, Germany)
using a 1D Gas detector detector
at a sample-detector distance of 2.3 m and
at a wavelength of λ = 0.15 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 3.5 and 3.9 mg/ml were measured
at 12°C.
15 successive
60 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
Storage temperature = UNKNOWN
Tags:
X33
|
|
||||||||||||||||||||||||||||||||||||||||||||||||