| MWexperimental | 16 | kDa |
| MWexpected | 15 | kDa |
| VPorod | 16 | nm3 |
|
log I(s)
3.17×10-2
3.17×10-3
3.17×10-4
3.17×10-5
|
s, nm-1
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The solution structures of higher-order human telomere G-quadruplex multimers.
Monsen RC, Chakravarthy S, Dean WL, Chaires JB, Trent JO, Nucleic Acids Res (2021) Europe PMCSASDKG3 – Human wild-type telomere 48-mer d(TTAGGG)8
Human Telomere Repeat (TTAGGG)8|
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Fits and models
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Synchrotron SAXS
data from solutions of
Human wild-type telomere 48-mer d(TTAGGG)8
in
6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH 7.2
were collected
on the
BioCAT 18ID beam line
at the Advanced Photon Source (APS), Argonne National Laboratory storage ring
(Lemont, IL, USA)
using a Pilatus3 X 1M detector
at a sample-detector distance of 3.5 m and
at a wavelength of λ = 0.1033 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 300.00 μl sample
at 13 mg/ml was injected at a 0.75 ml/min flow rate
onto a GE Superdex 75 Increase 10/300 column
at 20°C.
Four successive
0.500 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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