Functional Relevance of Interleukin-1 Receptor Inter-domain Flexibility for Cytokine Binding and Signaling.

Ge J, Remesh SG, Hammel M, Pan S, Mahan AD, Wang S, Wang X, Structure 27(8):1296-1307.e5 (2019) Europe PMC

SASDE89 – Interleukin-1 receptor accessory protein ectodomain with RII linker

Interleukin-1 receptor accessory protein ectodomains with RII linker
MWexperimental 50 kDa
MWexpected 41 kDa
VPorod 75 nm3
log I(s) 3.42×102 3.42×101 3.42×100 3.42×10-1
Interleukin-1 receptor accessory protein ectodomains with RII linker small angle scattering data  s, nm-1
ln I(s)
Interleukin-1 receptor accessory protein ectodomains with RII linker Guinier plot ln 3.43×102 Rg: 3.0 nm 0 (3.0 nm)-2 s2
(sRg)2I(s)/I(0)
Interleukin-1 receptor accessory protein ectodomains with RII linker Kratky plot 1.104 0 √3 sRg
p(r)
Interleukin-1 receptor accessory protein ectodomains with RII linker pair distance distribution function Rg: 3.1 nm 0 Dmax: 10.7 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Interleukin-1 receptor accessory protein ectodomains with RII linker BILBOMD model
Interleukin-1 receptor accessory protein ectodomains with RII linker BILBOMD model
Synchrotron SAXS data from solutions of Interleukin-1 receptor accessory protein ectodomain with RII linker in 10mM HEPES, 150mM NaCl, 3% glycerol, pH 7.2 were measured using size-exclusion chromatography SAXS (SEC-SAXS) on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.103 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). The data were collected as 600 successive 3 second frames through the SEC elution profile. Sample-elution peak data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.

SEC-SAXS was performed at 20°C using the following parameters: Column: Schodex kw-803 ; Flow rate: 0.5 mL/min; Total acquisition time: 30min; Sample injection concentration: 7.8 mg/mL; Injection volume: 50 μL.

Interleukin-1 receptor accessory protein ectodomains with RII linker
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   40.9 kDa
 
UniProt   Q9NPH3 (21-348)
Sequence   FASTA