Substrates and Cyclic Peptide Inhibitors of the Oligonucleotide-Activated Sirtuin 7.

Bolding JE, Nielsen AL, Jensen I, Hansen TN, Ryberg LA, Jameson ST, Harris P, Peters GHJ, Denu JM, Rogers JM, Olsen CA, Angew Chem Int Ed Engl :e202314597 (2023) Europe PMC

SASDTU2 – NAD-dependent protein deacetylase sirtuin-7 at pH 8.0

Isoform 1 of NAD-dependent protein deacetylase sirtuin-7
MWI(0) 42 kDa
MWexpected 45 kDa
VPorod 89 nm3
log I(s) 2.95×10-2 2.95×10-3 2.95×10-4 2.95×10-5
Isoform 1 of NAD-dependent protein deacetylase sirtuin-7 small angle scattering data  s, nm-1
ln I(s)
Isoform 1 of NAD-dependent protein deacetylase sirtuin-7 Guinier plot ln 2.95×10-2 Rg: 3.2 nm 0 (3.2 nm)-2 s2
(sRg)2I(s)/I(0)
Isoform 1 of NAD-dependent protein deacetylase sirtuin-7 Kratky plot 1.104 0 √3 sRg
p(r)
Isoform 1 of NAD-dependent protein deacetylase sirtuin-7 pair distance distribution function Rg: 3.3 nm 0 Dmax: 11.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Isoform 1 of NAD-dependent protein deacetylase sirtuin-7 CORAL model
log I(s)
 s, nm-1
Isoform 1 of NAD-dependent protein deacetylase sirtuin-7 ALPHAFOLD PROTEIN STRUCTURE DATABASE model
log I(s)
 s, nm-1
Isoform 1 of NAD-dependent protein deacetylase sirtuin-7 DAMMIN model
Synchrotron SAXS data from solutions of NAD-dependent protein deacetylase sirtuin-7 in 50 mM Tris-HCl, pH 8 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1 and 3.9 mg/ml were measured at 20°C. 30 successive 0.095 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Isoform 1 of NAD-dependent protein deacetylase sirtuin-7 (SIRT7)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   44.9 kDa
 
UniProt   Q9NRC8-1 (1-400)
Sequence   FASTA
 
ALPHAFOLD ID   Q9NRC8