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6 hits found for Lipid A export ATP-binding/permease protein MsbA

...ASDDB5 – MsbA in stealth nanodisc (SANS, 100% D2O)

Lipid A export ATP-binding/permease protein MsbAMembrane scaffold protein 1D1 (deuterated, 75%)1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) experimental SAS data
SASREF CV model
Sample: Lipid A export ATP-binding/permease protein MsbA dimer, 133 kDa ...a coli protein
...ane scaffold protein 1D1 (deuterated, 75%) dimer, 49 kDa protein
...almitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) , 1 kDa ...a coli
Buffer: ...aCl, pH: 7.5
Experiment: ...ANS data collected at D11, ILL on ...ar 9
...ational States of ABC Transporter MsbA in a Lipid Environment Investigated by Small-Angle Scattering Using Stealth Carrier Nanodiscs. Structure 26(8):1072-1079.e4 (2018)
...aric S, Mertens HD, Moulin M, Haertlein M, Prevost S, Svergun DI, Busch S, Forsyth VT, Tidow H
RgGuinier 4.0 nm
Dmax 13.0 nm
VolumePorod 189 nm3

...ASDDC5 – MsbA in stealth nanodisc (SANS, 100% D2O) + 1 mM ADP

Lipid A export ATP-binding/permease protein MsbAMembrane scaffold protein 1D1 (deuterated, 75%)1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) experimental SAS data
Lipid A export ATP-binding/permease protein MsbA Membrane scaffold protein 1D1 (deuterated, 75%) 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) Kratky plot
Sample: Lipid A export ATP-binding/permease protein MsbA dimer, 133 kDa ...a coli protein
...ane scaffold protein 1D1 (deuterated, 75%) dimer, 49 kDa protein
...almitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) , 1 kDa ...a coli
Buffer: ...aCl, 1 mM ADP, pH: 7.5
Experiment: ...ANS data collected at D11, ILL on ...ar 9
...ational States of ABC Transporter MsbA in a Lipid Environment Investigated by Small-Angle Scattering Using Stealth Carrier Nanodiscs. Structure 26(8):1072-1079.e4 (2018)
...aric S, Mertens HD, Moulin M, Haertlein M, Prevost S, Svergun DI, Busch S, Forsyth VT, Tidow H
RgGuinier 3.9 nm
Dmax 12.5 nm
VolumePorod 173 nm3

...ASDDD5 – MsbA in stealth nanodisc (SAXS)

Lipid A export ATP-binding/permease protein MsbAMembrane scaffold protein 1D1 (deuterated, 75%)1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) experimental SAS data
Lipid A export ATP-binding/permease protein MsbA Membrane scaffold protein 1D1 (deuterated, 75%) 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) Kratky plot
Sample: Lipid A export ATP-binding/permease protein MsbA dimer, 133 kDa ...a coli protein
...ane scaffold protein 1D1 (deuterated, 75%) dimer, 49 kDa protein
...almitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) , 1 kDa ...a coli
Buffer: ...aCl, pH: 7.5
Experiment: ...AXS data collected at EMBL P12, ...A III on 2017 Sep 8
...ational States of ABC Transporter MsbA in a Lipid Environment Investigated by Small-Angle Scattering Using Stealth Carrier Nanodiscs. Structure 26(8):1072-1079.e4 (2018)
...aric S, Mertens HD, Moulin M, Haertlein M, Prevost S, Svergun DI, Busch S, Forsyth VT, Tidow H
RgGuinier 4.8 nm
Dmax 16.0 nm
VolumePorod 607 nm3

...ASDDE5 – ...MsbA (apo)

Nucleotide Binding Domain of Lipid A export ATP-binding/permease protein MsbA experimental SAS data
Nucleotide Binding Domain of Lipid A export ATP-binding/permease protein MsbA Kratky plot
Sample: ...ain of Lipid A export ATP-binding/permease protein MsbA monomer, 27 kDa ...a coli protein
Buffer: ...aCl, 0.5 mM TCEP, pH: 7.5
Experiment: ...AXS data collected at EMBL P12, ...A III on ...ay 30
...ational States of ABC Transporter MsbA in a Lipid Environment Investigated by Small-Angle Scattering Using Stealth Carrier Nanodiscs. Structure 26(8):1072-1079.e4 (2018)
...aric S, Mertens HD, Moulin M, Haertlein M, Prevost S, Svergun DI, Busch S, Forsyth VT, Tidow H
RgGuinier 2.2 nm
Dmax 7.3 nm
VolumePorod 47 nm3

...ASDDF5 – ...MsbA (+1mM ADP)

Nucleotide Binding Domain of Lipid A export ATP-binding/permease protein MsbA experimental SAS data
Nucleotide Binding Domain of Lipid A export ATP-binding/permease protein MsbA Kratky plot
Sample: ...ain of Lipid A export ATP-binding/permease protein MsbA monomer, 27 kDa ...a coli protein
Buffer: ...aCl, 0.5 mM TCEP, 1 mM ADP, pH: 7.5
Experiment: ...AXS data collected at EMBL P12, ...A III on ...ay 30
...ational States of ABC Transporter MsbA in a Lipid Environment Investigated by Small-Angle Scattering Using Stealth Carrier Nanodiscs. Structure 26(8):1072-1079.e4 (2018)
...aric S, Mertens HD, Moulin M, Haertlein M, Prevost S, Svergun DI, Busch S, Forsyth VT, Tidow H
RgGuinier 2.1 nm
Dmax 7.3 nm
VolumePorod 50 nm3

...ASDGV5 – ...ain of Lipid A export ATP-binding/permease protein MsbA - data from stop-and-flow time-resolved SAXS (12 s time course)

Lipid A export ATP-binding/permease protein MsbA - Nucleotide binding domain experimental SAS data
Lipid A export ATP-binding/permease protein MsbA - Nucleotide binding domain Kratky plot
Sample: Lipid A export ATP-binding/permease protein MsbA - Nucleotide binding domain monomer, 27 kDa ...a coli protein
Buffer: ...aCl, 5 mM MgCl2, 0.45 mM Mg2+-ATP, pH: 7.5
Experiment: ...AXS data collected at EMBL P12, ...A III on 2017 Dec 8
...al Kinetics of MsbA Investigated by Stopped-Flow Time-Resolved Small-Angle X-Ray Scattering. Structure (2019)
...ao Y, Monteiro DCF, Niebling S, Nitsche J, Veith K, Gräwert TW, Blanchet CE, Schroer MA, Huse N, Pearson AR, Svergun DI, Tidow H
RgGuinier 2.1 nm
Dmax 6.8 nm
VolumePorod 50 nm3