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2 hits found for Multidrug resistance operon repressor

SASDMG9 – Multidrug resistance operon repressor (MexR) of the MexAB-OprM multidrug efflux pump operon of Pseudomonas aeruginosa

Multidrug resistance operon repressor experimental SAS data
DAMFILT model
Sample: Multidrug resistance operon repressor dimer, 32 kDa Pseudomonas aeruginosa protein
Buffer: 20mM HEPES, 150mM NaCl, 10mM DTT, 1% v/v glycerol, pH: 7.1
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Nov 23
Small-angle X-ray and neutron scattering of MexR and its complex with DNA supports a conformational selection binding model Biophysical Journal (2022)
Caporaletti F, Pietras Z, Morad V, Mårtensson L, Gabel F, Wallner B, Martel A, Sunnerhagen M
RgGuinier 2.3 nm
Dmax 7.7 nm
VolumePorod 56 nm3

SASDMH9 – ...Multidrug resistance operon repressor (MexR) in complex with 34bp dsDNA binding sequence (SANS data at 0% D2O)

Multidrug resistance operon repressor34 base pair double-stranded DNA experimental SAS data
MONSA model
Sample: Multidrug resistance operon repressor dimer, 32 kDa Pseudomonas aeruginosa protein
34 base pair double-stranded DNA monomer, 21 kDa synthetic construct DNA
Buffer: 20mM NaPO4, 150 mM NaCl, 10 mM DTT, pH: 7.1
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 May 30
Small-angle X-ray and neutron scattering of MexR and its complex with DNA supports a conformational selection binding model Biophysical Journal (2022)
Caporaletti F, Pietras Z, Morad V, Mårtensson L, Gabel F, Wallner B, Martel A, Sunnerhagen M
RgGuinier 2.9 nm
Dmax 7.8 nm
VolumePorod 79 nm3