SidF, a dual substrate N5-acetyl-N5-hydroxy-L-ornithine transacetylase involved in Aspergillus fumigatus siderophore biosynthesis

Poonsiri T, Stransky J, Demitri N, Haas H, Cianci M, Benini S, Journal of Structural Biology: X 11:100119 (2025) DOI

SASDVB8 – C-terminal truncation mutant of N(5)-hydroxyornithine:cis-anhydromevalonyl coenzyme A-N(5)-transacylase sidF

N(5)-hydroxyornithine:cis-anhydromevalonyl coenzyme A-N(5)-transacylase sidF (Δ444-462)
MWexperimental 109 kDa
MWexpected 107 kDa
VPorod 189 nm3
log I(s) 2.76×10-1 2.76×10-2 2.76×10-3 2.76×10-4
N(5)-hydroxyornithine:cis-anhydromevalonyl coenzyme A-N(5)-transacylase sidF (Δ444-462) small angle scattering data  s, nm-1
ln I(s)
N(5)-hydroxyornithine:cis-anhydromevalonyl coenzyme A-N(5)-transacylase sidF (Δ444-462) Guinier plot ln 2.77×10-1 Rg: 3.5 nm 0 (3.5 nm)-2 s2
(sRg)2I(s)/I(0)
N(5)-hydroxyornithine:cis-anhydromevalonyl coenzyme A-N(5)-transacylase sidF (Δ444-462) Kratky plot 1.104 0 √3 sRg
p(r)
N(5)-hydroxyornithine:cis-anhydromevalonyl coenzyme A-N(5)-transacylase sidF (Δ444-462) pair distance distribution function Rg: 3.7 nm 0 Dmax: 15 nm

Data validation

Fits and models

log I(s)
 s, nm-1
N(5)-hydroxyornithine:cis-anhydromevalonyl coenzyme A-N(5)-transacylase sidF (Δ444-462) CORAL model
SAXS data from solutions of C-terminal truncated sidF in 50 mM Tris, 200 mM NaCl, pH 8 were collected using an Anton Paar SAXSpoint 2.0 instrument at the Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure (Vestec, Czech Republic) equipped with an Eiger R 1M detector at a sample-detector distance of 0.6 m and at a wavelength of λ = 0.134 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). A sample concentration at 5 mg/ml was measured at 20°C. 60 successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

N(5)-hydroxyornithine:cis-anhydromevalonyl coenzyme A-N(5)-transacylase sidF (Δ444-462) (SidF C-trim)
Mol. type   Protein
Organism   Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293)
Olig. state   Dimer
Mon. MW   53.5 kDa
 
UniProt   Q4WF55 (1-443)
Sequence   FASTA