| MWI(0) | 26 | kDa |
| MWexpected | 20 | kDa |
| VPorod | 46 | nm3 |
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log I(s)
8.20×10-3
8.20×10-4
8.20×10-5
8.20×10-6
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s, nm-1
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Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with α-actinin.
Sponga A, Arolas JL, Schwarz TC, Jeffries CM, Rodriguez Chamorro A, Kostan J, Ghisleni A, Drepper F, Polyansky A, De Almeida Ribeiro E, Pedron M, Zawadzka-Kazimierczuk A, Mlynek G, Peterbauer T, Doto P, Schreiner C, Hollerl E, Mateos B, Geist L, Faulkner G, Kozminski W, Svergun DI, Warscheid B, Zagrovic B, Gautel M, Konrat R, Djinović-Carugo K, Sci Adv 7(22) (2021) Europe PMCSASDJJ6 – Sarcomeric intrinsically disordered protein FATZ-1 (N-FATZ-1)
N-ter construct of FATZ-1 (alias myozenin-1 or calsarcin-2)|
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Fits and models
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Rg, nm
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Synchrotron SAXS data from solutions of N-FATZ-1 in 50 mM Tris-HCl 150 mM NaCl, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 40.00 μl of sample was injected at a 0.30 ml/min flow rate onto a GE Superose 6 Increase 5/150 column at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Three SEC-SAXS runs were averaged to generate the dataset displayed in this entry. The three individual and averaged SEC-SAXS profiles (obtained from 67-100 0.25 s exposures through the major SEC-elution peak using either 4.7 or 2.3 mg/ml sample injection concentrations) are included in the full entry zip archive. The MW estimate from parallel MALLS-RI measurements is 22 kDa.
Tags:
idp
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