| MWexperimental | 240 | kDa |
| MWexpected | 250 | kDa |
| VPorod | 470 | nm3 |
|
log I(s)
4.71×10-1
4.71×10-2
4.71×10-3
4.71×10-4
|
s, nm-1
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A mechanism for histone chaperoning activity of nucleoplasmin: thermodynamic and structural models.
Taneva SG, Bañuelos S, Falces J, Arregi I, Muga A, Konarev PV, Svergun DI, Velázquez-Campoy A, Urbaneja MA, J Mol Biol 393(2):448-63 (2009) Europe PMCSASDA75 – Nucleoplasmin-H2AH2B complex
NP-H2AH2B|
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Fits and models
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Synchrotron SAXS
data from solutions of
Nucleoplasmin-H2AH2B complex
in
20 mM Pipes buffer 150 mM NaCl, pH 7.5
were collected
on the
EMBL X33 beam line
at the DORIS III, DESY storage ring
(Hamburg, Germany)
using a Pilatus 1M-W detector
at a sample-detector distance of 2.7 m and
at a wavelength of λ = 0.15 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 3 and 5 mg/ml were measured
at 10°C.
Four successive
15 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Tags:
X33
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