Standard polystyrene nanospheres (concentration series data)

Dima Molodenskiy.

SASDMW3 – Thermo Fisher polystyrene nanospheres with a diameter of 20 nm

Nominal 20 nm diameter polystyrene spheres
MWexperimental 1 kDa
MWexpected 8 kDa
VPorod 3 nm3
log I(s) 5.99×10-3 5.99×10-4 5.99×10-5 5.99×10-6
Nominal 20 nm diameter polystyrene spheres small angle scattering data  s, nm-1
ln I(s)
Nominal 20 nm diameter polystyrene spheres Guinier plot ln 6.00×10-3 Rg: 8.5 nm 0 (8.5 nm)-2 s2
(sRg)2I(s)/I(0)
Nominal 20 nm diameter polystyrene spheres Kratky plot 1.104 0 √3 sRg
p(r)
Nominal 20 nm diameter polystyrene spheres pair distance distribution function Rg: 8.7 nm 0 Dmax: 25 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Nominal 20 nm diameter polystyrene spheres BODIES model
log I(s)
 s, nm-1
Nominal 20 nm diameter polystyrene spheres DAMMIF model
Synchrotron SAXS data from solutions of Thermo Fisher polystyrene nanospheres with a diameter of 20 nm in Water, pH 7 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 6.00 mg/ml was measured at 20°C. 50 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Nominal 20 nm diameter polystyrene spheres (Polystyrene spheres)
Mol. type   Other
Olig. state   0
Mon. MW   0.1 kDa
Chemical formula