| MWexperimental | 106 | kDa |
| MWexpected | 100 | kDa |
| VPorod | 138 | nm3 |
|
log I(s)
1.50×101
1.50×100
1.50×10-1
1.50×10-2
|
s, nm-1
|
Insulin Binding to the Analytical Antibody Sandwich Pair OXI
‐005 and HUI
‐018 – Epitope Mapping and Binding Properties
Johansson E,
Wu X,
Yu B,
Yang Z,
Cao Z,
Wiberg C,
Jeppesen C,
Poulsen F,
Protein Science
(2020)
DOISASDJ24 – Immunoglobulin HUI-018 Fab and OXI-005 Fab in a ternary complex with porcine insulin
HUI-018 FabInsulin (Insulin B chain and Insulin A chain)
OXI-005 Fab
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Fits and models
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SAXS data from solutions of HUI-018 Fab and OXI-005 Fab in a ternary complex with porcine insulin in 20 mM HEPES, 140 mM NaCl, pH 7.5 were collected using a Rigaku BioSAXS-2000 equipped with a Pilatus 100K detector (Novo Nordisk A/S, Maaloev, Denmark) at a wavelength of λ = 0.15418 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 20°C. Six successive 600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Sample detector distance = UNKNOWN |
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