The N-terminus of obscurin is flexible in solution.

Mauriello GE, Moncure GE, Nowzari RA, Miller CJ, Wright NT, Proteins (2022) Europe PMC

SASDNX7 – Obscurin Ig domains 12/13, long at neutral pH

Obscurin Ig domains 12/13 long
MWexperimental 21 kDa
MWexpected 22 kDa
VPorod 26 nm3
log I(s) 1.64×100 1.64×10-1 1.64×10-2 1.64×10-3
Obscurin Ig domains 12/13 long small angle scattering data  s, nm-1
ln I(s)
Obscurin Ig domains 12/13 long Guinier plot ln 1.65×100 Rg: 2.9 nm 0 (2.9 nm)-2 s2
(sRg)2I(s)/I(0)
Obscurin Ig domains 12/13 long Kratky plot 1.104 0 3 sRg
Dmax: 10 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Obscurin Ig domains 12/13 long MULTIFOXS model

Synchrotron SAXS data from solutions of Obscurin Ig domains 12/13, long at neutral pH in 20 mM Tris, 50 mM NaCl, 0.35 mM NaN3, pH 7.5 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS) storage ring (Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 8.00 mg/ml was measured. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

X-ray exposure time: UNKNOWN.

Obscurin Ig domains 12/13 long (Obscurin Ig 12/13 +)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   21.5 kDa
 
UniProt   Q5VST9 (1071-1254)
Sequence   FASTA