Structural studies of Old Yellow Enzyme of Leishmania braziliensis in solution.

Walbert Veloso-Silva LL, Dores-Silva PR, Bertolino Reis DE, Moreno Oliveira LF, Libardi SH, Borges JC, Arch Biochem Biophys (2018) Europe PMC

SASDET5 – Old Yellow Enzyme of Leishmania braziliensis

Old Yellow Enzyme of Leishmania braziliensis
MWI(0) 49 kDa
MWexpected 42 kDa
VPorod 73 nm3
log I(s) 3.81×10-1 3.81×10-2 3.81×10-3 3.81×10-4
Old Yellow Enzyme of Leishmania braziliensis small angle scattering data  s, nm-1
ln I(s)
Old Yellow Enzyme of Leishmania braziliensis Guinier plot ln 3.81×10-1 Rg: 2.7 nm 0 (2.7 nm)-2 s2
(sRg)2I(s)/I(0)
Old Yellow Enzyme of Leishmania braziliensis Kratky plot 1.104 0 √3 sRg
p(r)
Old Yellow Enzyme of Leishmania braziliensis pair distance distribution function Rg: 2.7 nm 0 Dmax: 9.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Old Yellow Enzyme of Leishmania braziliensis DAMMIN model
Synchrotron SAXS data from solutions of old yellow enzyme in 25 mM Tris-HCl 100 mM NaCl and 1 mM β-mercaptoethanol, pH 8 were collected on the SAXS1 beam line at the Brazilian Synchrotron Light Laboratory (Campinas, Brazil) using a 20Hz Pilatus 300K detector at a sample-detector distance of 1 m and at a wavelength of λ = 0.148 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 25°C. One 360 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Old Yellow Enzyme of Leishmania braziliensis (LbOYE)
Mol. type   Protein
Organism   Leishmania braziliensis
Olig. state   Monomer
Mon. MW   41.9 kDa
Sequence   FASTA