NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin.

Tants JN, Becker LM, McNicoll F, Müller-McNicoll M, Schlundt A, Nucleic Acids Res (2022) Europe PMC

SASDN96 – Ox40 RNA (untranslated region, UTR) Bulge with alternative and constitutive decay elements (Bulge-ADE-CDE)

Ox40 3 UTR Bulge-ADE-CDE
MWexperimental 32 kDa
MWexpected 35 kDa
VPorod 46 nm3
log I(s) 7.97×10-2 7.97×10-3 7.97×10-4 7.97×10-5
Ox40 3 UTR Bulge-ADE-CDE small angle scattering data  s, nm-1
ln I(s)
Ox40 3 UTR Bulge-ADE-CDE Guinier plot ln 7.98×10-2 Rg: 3.4 nm 0 (3.4 nm)-2 s2
(sRg)2I(s)/I(0)
Ox40 3 UTR Bulge-ADE-CDE Kratky plot 1.104 0 3 sRg
Dmax: 11 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of Ox40 RNA (untranslated region, UTR) Bulge with alternative and constitutive decay elements (Bulge-ADE-CDE) in 50 mM KCl, 25 mM sodium phosphate, pH 7 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.28 mg/ml was measured at 20.1°C. 15 successive 0.145 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Ox40 3 UTR Bulge-ADE-CDE
Mol. type   RNA
Organism   Mus musculus
Olig. state   Monomer
Mon. MW   35.2 kDa
Sequence   FASTA