| MWexperimental | 76 | kDa |
| MWexpected | 65 | kDa |
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log I(s)
1.32×104
1.32×103
1.32×102
1.32×101
|
s, nm-1
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Structural variability of EspG chaperones from mycobacterial ESX-1, ESX-3 and ESX-5 type VII secretion systems
Tuukkanen A, Freire D, Chan S, Arbing M, Reed R, Evans T, Zenkeviciutė G, Kim J, Kahng S, Sawaya M, Chaton C, Wilmanns M, Eisenberg D, Parret A, Korotkov K, (2018) DOISASDDW2 – EspG5-PE25/PPE41 complex from M. tuberculosis
EspG5 chaperone from Mycobacterium tuberculosisPE25 from Mycobacterium tuberculosis
PPE41 from Mycobacterium tuberculosis
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Fits and models
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Synchrotron SAXS
data from solutions of
EspG5-PE25/PPE41 complex from M. tuberculosis
in
20 mM HEPES pH 7.5, 150 mM NaCl, pH 7.5
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 2M detector
at a wavelength of λ = 0.124 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 0.48 mg/ml was measured.
20 successive
0.050 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Cell temperature = UNKNOWN. Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN |
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