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4 hits found for Piwi protein AF_1318

SASDJ75 – ...protein

Piwi protein AF_1318 experimental SAS data
OTHER model
Sample: Piwi protein AF_1318 dimer, 98 kDa Archaeoglobus fulgidus protein
Buffer: 20 mM TrisHCl, pH 7.5, 5 mM MgCl2, 500 mM NaCl and 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 1
...protein dimerises in solution
Elena Manakova
RgGuinier 3.8 nm
Dmax 12.1 nm
VolumePorod 174 nm3

SASDH39 – Complex of Archaeoglobus fulgidus Argonaute with dsDNA (5'p-ATTGTACGTACAAT)

Piwi protein AF_13185'-phosphorilated 14-mer DNA oligoduplex experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Piwi protein AF_1318 dimer, 102 kDa Archaeoglobus fulgidus protein
5'-phosphorilated 14-mer DNA oligoduplex dimer, 9 kDa DNA
Buffer: 20 mM TrisHCl, pH 7.5, 5 mM MgCl2, 150 mM NaCl and 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 6
Prokaryotic Argonaute from Archaeoglobus fulgidus interacts with DNA as a homodimer. Sci Rep 11(1):4518 (2021)
Golovinas E, Rutkauskas D, Manakova E, Jankunec M, Silanskas A, Sasnauskas G, Zaremba M
RgGuinier 3.2 nm
Dmax 10.1 nm
VolumePorod 158 nm3

SASDH49 – Complex of Archaeoglobus fulgidus Argonaute monomeric mutant with dsDNA (5'p-ATTGTACGTACAAT)

Piwi protein AF_1318 delta (296-303) mutant5'-phosphorilated 14-mer DNA oligoduplex experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Piwi protein AF_1318 delta (296-303) mutant monomer, 50 kDa Archaeoglobus fulgidus protein
5'-phosphorilated 14-mer DNA oligoduplex dimer, 9 kDa DNA
Buffer: 20 mM TrisHCl, pH 7.5, 5 mM MgCl2, 150 mM NaCl and 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 6
Prokaryotic Argonaute from Archaeoglobus fulgidus interacts with DNA as a homodimer. Sci Rep 11(1):4518 (2021)
Golovinas E, Rutkauskas D, Manakova E, Jankunec M, Silanskas A, Sasnauskas G, Zaremba M
RgGuinier 2.8 nm
Dmax 9.6 nm
VolumePorod 109 nm3

SASDRX8 – ...protein Af1318 and AfAgo-N protein (containing N-L1-L2 domains) with a 14 base pair DNA oligoduplex in low salt buffer

Piwi protein AF_1318 (Archaeoglobus fulgidus AfAgo protein)Uncharacterized protein (AfAgo-N protein containing N-L1-L2 domains)5'-end phosphorylated DNA oligoduplex, 14 bp (MZ1288) experimental SAS data
CUSTOM IN-HOUSE model
Sample: Piwi protein AF_1318 (Archaeoglobus fulgidus AfAgo protein) monomer, 51 kDa Archaeoglobus fulgidus (strain … protein
...protein (AfAgo-N protein containing N-L1-L2 domains) monomer, 31 kDa Archaeoglobus fulgidus DSM … protein
5'-end phosphorylated DNA oligoduplex, 14 bp (MZ1288) monomer, 9 kDa DNA
Buffer: 20 mM TrisHCl pH 7.5, 200 mM NaCl, 5 mM MgCl2, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 6
...protein. Nucleic Acids Res (2024)
Manakova E, Golovinas E, Pocevičiūtė R, Sasnauskas G, Silanskas A, Rutkauskas D, Jankunec M, Zagorskaitė E, Jurgelaitis E, Grybauskas A, Venclovas Č, Zaremba M
RgGuinier 2.9 nm
Dmax 9.7 nm
VolumePorod 145 nm3