4 hits found for Piwi protein AF_1318 (Archaeoglobus fulgidus AfAgo protein)

SASDJ75 – ...fulgidus Argonaute protein Sample: Piwi protein AF_1318 dimer, 98 kDa ...fulgidus protein Buffer: 20 mM TrisHCl, pH 7.5, 5 mM MgCl2, 500 mM NaCl and 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 1 ...fulgidus Argonaute protein dimerises in solution Elena Manakova RgGuinier 3.8 nm Dmax 12.1 nm VolumePorod 174 nm3

SASDH39 – ...fulgidus Argonaute with dsDNA (5'p-ATTGTACGTACAAT) Sample: Piwi protein AF_1318 dimer, 102 kDa ...fulgidus protein 5'-phosphorilated 14-mer DNA oligoduplex dimer, 9 kDa DNA Buffer: 20 mM TrisHCl, pH 7.5, 5 mM MgCl2, 150 mM NaCl and 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 6 ...fulgidus interacts with DNA as a homodimer. Sci Rep 11(1):4518 (2021) Golovinas E, Rutkauskas D, Manakova E, Jankunec M, Silanskas A, Sasnauskas G, Zaremba M RgGuinier 3.2 nm Dmax 10.1 nm VolumePorod 158 nm3

SASDH49 – ...fulgidus Argonaute monomeric mutant with dsDNA (5'p-ATTGTACGTACAAT) Sample: Piwi protein AF_1318 delta (296-303) mutant monomer, 50 kDa ...fulgidus protein 5'-phosphorilated 14-mer DNA oligoduplex dimer, 9 kDa DNA Buffer: 20 mM TrisHCl, pH 7.5, 5 mM MgCl2, 150 mM NaCl and 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 6 ...fulgidus interacts with DNA as a homodimer. Sci Rep 11(1):4518 (2021) Golovinas E, Rutkauskas D, Manakova E, Jankunec M, Silanskas A, Sasnauskas G, Zaremba M RgGuinier 2.8 nm Dmax 9.6 nm VolumePorod 109 nm3

SASDRX8 – ...AfAgo protein Af1318 and AfAgo-N protein (containing N-L1-L2 domains) with a 14 base pair DNA oligoduplex in low salt buffer Sample: Piwi protein AF_1318 (Archaeoglobus fulgidus AfAgo protein) monomer, 51 kDa ...fulgidus (strain … protein ...protein (AfAgo-N protein containing N-L1-L2 domains) monomer, 31 kDa ...fulgidus DSM … protein 5'-end phosphorylated DNA oligoduplex, 14 bp (MZ1288) monomer, 9 kDa DNA Buffer: 20 mM TrisHCl pH 7.5, 200 mM NaCl, 5 mM MgCl2, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 6 ...fulgidus Argonaute forms a functional heterodimer with an N-L1-L2 domain protein. Nucleic Acids Res (2024) Manakova E, Golovinas E, Pocevičiūtė R, Sasnauskas G, Silanskas A, Rutkauskas D, Jankunec M, Zagorskaitė E, Jurgelaitis E, Grybauskas A, Venclovas Č, Zaremba M RgGuinier 2.9 nm Dmax 9.7 nm VolumePorod 145 nm3