DPY30 acts as an ASH2L-specific stabilizer to stimulate the enzyme activity of MLL family methyltransferases on different substrates.

Zhao L, Huang N, Mencius J, Li Y, Xu Y, Zheng Y, He W, Li N, Zheng J, Zhuang M, Quan S, Chen Y, iScience 25(9):104948 (2022) Europe PMC

SASDPU3 – Histone-lysine N-methyltransferase multi protein complex bound to protein dpy-30 homologue: MLL1-WDR5-RBBP5-ASH2L-DPY30

Histone-lysine N-methyltransferase 2A
WD repeat-containing protein 5
Retinoblastoma-binding protein 5
Set1/Ash2 histone methyltransferase complex subunit ASH2
Protein dpy-30 homolog
MWexperimental 208 kDa
MWexpected 203 kDa
VPorod 411 nm3
log I(s) 1.95×102 1.95×101 1.95×100 1.95×10-1
Histone-lysine N-methyltransferase 2A WD repeat-containing protein 5 Retinoblastoma-binding protein 5 Set1/Ash2 histone methyltransferase complex subunit ASH2 Protein dpy-30 homolog small angle scattering data  s, nm-1
ln I(s)
Histone-lysine N-methyltransferase 2A WD repeat-containing protein 5 Retinoblastoma-binding protein 5 Set1/Ash2 histone methyltransferase complex subunit ASH2 Protein dpy-30 homolog Guinier plot ln 1.96×102 Rg: 5.4 nm 0 (5.4 nm)-2 s2
(sRg)2I(s)/I(0)
Histone-lysine N-methyltransferase 2A WD repeat-containing protein 5 Retinoblastoma-binding protein 5 Set1/Ash2 histone methyltransferase complex subunit ASH2 Protein dpy-30 homolog Kratky plot 1.104 0 3 sRg
p(r)
Histone-lysine N-methyltransferase 2A WD repeat-containing protein 5 Retinoblastoma-binding protein 5 Set1/Ash2 histone methyltransferase complex subunit ASH2 Protein dpy-30 homolog pair distance distribution function Rg: 5.5 nm 0 Dmax: 17.5 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of MLL1-WDR5-RBBP5-ASH2L-DPY30 in 25 mM HEPES, 150 mM NaCl, 1 mM TECP, pH 7.4 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facility (SSRF, Shanghai, China) using a Pilatus 1M detector at a sample-detector distance of 2.4 m and at a wavelength of λ = 0.09184 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 10°C. 20 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Histone-lysine N-methyltransferase 2A (MLL1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   24.9 kDa
 
UniProt   Q03164 (3754-3969)
Sequence   FASTA
 
WD repeat-containing protein 5 (WDR5)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   36.6 kDa
 
UniProt   P61964 (1-334)
Sequence   FASTA
 
Retinoblastoma-binding protein 5 (RBBP5)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   59.2 kDa
 
UniProt   Q15291 (1-538)
Sequence   FASTA
 
Set1/Ash2 histone methyltransferase complex subunit ASH2 (ASH2L)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   60.2 kDa
 
UniProt   Q9UBL3-3 (95-534)
Sequence   FASTA
 
Protein dpy-30 homolog (DPY30)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   11.3 kDa
 
UniProt   Q9C005 (1-99)
Sequence   FASTA