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6 hits found for Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2)

SASDP56 – ...protein 8 (nsp8, dimer) in 250 mM NaCl buffer

Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) experimental SAS data
Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) Kratky plot
Sample: Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) dimer, 44 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES pH 7.4, 250 mM NaCl, pH: 7.4
Experiment: SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19
Multiscale characterization reveals oligomerization dependent phase separation of primer-independent RNA polymerase nsp8 from SARS-CoV-2. Commun Biol 5(1):925 (2022)
Xu J, Jiang X, Zhang Y, Dong Y, Ma C, Jiang H, Zuo T, Chen R, Ke Y, Cheng H, Wang H, Liu J
RgGuinier 4.1 nm

SASDP66 – ...protein 8 (nsp8, dimer) in 500 mM NaCl buffer

Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) experimental SAS data
Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) Kratky plot
Sample: Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) dimer, 44 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES pH 7.4, 500 mM NaCl, pH: 7.4
Experiment: SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19
Multiscale characterization reveals oligomerization dependent phase separation of primer-independent RNA polymerase nsp8 from SARS-CoV-2. Commun Biol 5(1):925 (2022)
Xu J, Jiang X, Zhang Y, Dong Y, Ma C, Jiang H, Zuo T, Chen R, Ke Y, Cheng H, Wang H, Liu J
RgGuinier 3.8 nm
Dmax 14.0 nm
VolumePorod 98 nm3

SASDP76 – ...protein 8 (nsp8, dimer) in 1000 mM NaCl buffer

Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) experimental SAS data
Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) Kratky plot
Sample: Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) dimer, 44 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES pH 7.4, 1000 mM NaCl, pH: 7.4
Experiment: SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19
Multiscale characterization reveals oligomerization dependent phase separation of primer-independent RNA polymerase nsp8 from SARS-CoV-2. Commun Biol 5(1):925 (2022)
Xu J, Jiang X, Zhang Y, Dong Y, Ma C, Jiang H, Zuo T, Chen R, Ke Y, Cheng H, Wang H, Liu J
RgGuinier 3.8 nm
Dmax 14.0 nm
VolumePorod 100 nm3

SASDP86 – ...protein 8 (nsp8, tetramer) in 250 mM NaCl buffer

Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) experimental SAS data
Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) Kratky plot
Sample: Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) tetramer, 88 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES pH 7.4, 250 mM NaCl, pH: 7.4
Experiment: SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19
Multiscale characterization reveals oligomerization dependent phase separation of primer-independent RNA polymerase nsp8 from SARS-CoV-2. Commun Biol 5(1):925 (2022)
Xu J, Jiang X, Zhang Y, Dong Y, Ma C, Jiang H, Zuo T, Chen R, Ke Y, Cheng H, Wang H, Liu J
RgGuinier 6.3 nm
Dmax 24.0 nm

SASDP96 – ...protein 8 (nsp8, tetramer) in 500 mM NaCl buffer

Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) experimental SAS data
Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) Kratky plot
Sample: Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) tetramer, 88 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES pH 7.4, 500 mM NaCl, pH: 7.4
Experiment: SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19
Multiscale characterization reveals oligomerization dependent phase separation of primer-independent RNA polymerase nsp8 from SARS-CoV-2. Commun Biol 5(1):925 (2022)
Xu J, Jiang X, Zhang Y, Dong Y, Ma C, Jiang H, Zuo T, Chen R, Ke Y, Cheng H, Wang H, Liu J
RgGuinier 5.2 nm
Dmax 18.0 nm

SASDPA6 – ...protein 8 (nsp8, tetramer) in 1000 mM NaCl buffer

Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) experimental SAS data
Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) Kratky plot
Sample: Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) tetramer, 88 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES pH 7.4, 1000 mM NaCl, pH: 7.4
Experiment: SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19
Multiscale characterization reveals oligomerization dependent phase separation of primer-independent RNA polymerase nsp8 from SARS-CoV-2. Commun Biol 5(1):925 (2022)
Xu J, Jiang X, Zhang Y, Dong Y, Ma C, Jiang H, Zuo T, Chen R, Ke Y, Cheng H, Wang H, Liu J
RgGuinier 4.8 nm
Dmax 16.0 nm