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8 hits found for Replicase polyprotein 1a - nsp8

SASDKJ4 – ...nsp8

Replicase polyprotein 1a - nsp7Replicase polyprotein 1a - nsp8 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Replicase polyprotein 1a - nsp7 , 18 kDa Severe acute respiratory … protein
Replicase polyprotein 1a - nsp8 , 44 kDa Severe acute respiratory … protein
Buffer: 50 mM Tris, pH 8.0, 100 mM NaCl, 4 mM DTT, 4 mM MgCl2, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 19
Hallmarks of Alpha- and Betacoronavirus non-structural protein 7+8 complexes Science Advances 7(10):eabf1004 (2021)
Krichel B, Bylapudi G, Schmidt C, Blanchet C, Schubert R, Brings L, Koehler M, Zenobi R, Svergun D, Lorenzen K, Madhugiri R, Ziebuhr J, Uetrecht C
RgGuinier 3.4 nm
Dmax 13.5 nm
VolumePorod 92 nm3

SASDP56 – ...nsp8, dimer) in 250 mM NaCl buffer

Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) experimental SAS data
Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) Kratky plot
Sample: Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) dimer, 44 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES pH 7.4, 250 mM NaCl, pH: 7.4
Experiment: SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19
...nsp8 from SARS-CoV-2. Commun Biol 5(1):925 (2022)
Xu J, Jiang X, Zhang Y, Dong Y, Ma C, Jiang H, Zuo T, Chen R, Ke Y, Cheng H, Wang H, Liu J
RgGuinier 4.1 nm

SASDP66 – ...nsp8, dimer) in 500 mM NaCl buffer

Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) experimental SAS data
Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) Kratky plot
Sample: Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) dimer, 44 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES pH 7.4, 500 mM NaCl, pH: 7.4
Experiment: SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19
...nsp8 from SARS-CoV-2. Commun Biol 5(1):925 (2022)
Xu J, Jiang X, Zhang Y, Dong Y, Ma C, Jiang H, Zuo T, Chen R, Ke Y, Cheng H, Wang H, Liu J
RgGuinier 3.8 nm
Dmax 14.0 nm
VolumePorod 98 nm3

SASDP76 – ...nsp8, dimer) in 1000 mM NaCl buffer

Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) experimental SAS data
Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) Kratky plot
Sample: Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) dimer, 44 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES pH 7.4, 1000 mM NaCl, pH: 7.4
Experiment: SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19
...nsp8 from SARS-CoV-2. Commun Biol 5(1):925 (2022)
Xu J, Jiang X, Zhang Y, Dong Y, Ma C, Jiang H, Zuo T, Chen R, Ke Y, Cheng H, Wang H, Liu J
RgGuinier 3.8 nm
Dmax 14.0 nm
VolumePorod 100 nm3

SASDP86 – ...nsp8, tetramer) in 250 mM NaCl buffer

Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) experimental SAS data
Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) Kratky plot
Sample: Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) tetramer, 88 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES pH 7.4, 250 mM NaCl, pH: 7.4
Experiment: SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19
...nsp8 from SARS-CoV-2. Commun Biol 5(1):925 (2022)
Xu J, Jiang X, Zhang Y, Dong Y, Ma C, Jiang H, Zuo T, Chen R, Ke Y, Cheng H, Wang H, Liu J
RgGuinier 6.3 nm
Dmax 24.0 nm

SASDP96 – ...nsp8, tetramer) in 500 mM NaCl buffer

Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) experimental SAS data
Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) Kratky plot
Sample: Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) tetramer, 88 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES pH 7.4, 500 mM NaCl, pH: 7.4
Experiment: SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19
...nsp8 from SARS-CoV-2. Commun Biol 5(1):925 (2022)
Xu J, Jiang X, Zhang Y, Dong Y, Ma C, Jiang H, Zuo T, Chen R, Ke Y, Cheng H, Wang H, Liu J
RgGuinier 5.2 nm
Dmax 18.0 nm

SASDPA6 – ...nsp8, tetramer) in 1000 mM NaCl buffer

Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) experimental SAS data
Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) Kratky plot
Sample: Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) tetramer, 88 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES pH 7.4, 1000 mM NaCl, pH: 7.4
Experiment: SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19
...nsp8 from SARS-CoV-2. Commun Biol 5(1):925 (2022)
Xu J, Jiang X, Zhang Y, Dong Y, Ma C, Jiang H, Zuo T, Chen R, Ke Y, Cheng H, Wang H, Liu J
RgGuinier 4.8 nm
Dmax 16.0 nm

SASDMK9 – Nonstructural Proteins 7 and 8 of Feline Coronavirus (complex 1:2)

Replicase polyprotein 1aReplicase polyprotein 1a experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Replicase polyprotein 1a dimer, 19 kDa Severe acute respiratory … protein
Replicase polyprotein 1a monomer, 22 kDa Severe acute respiratory … protein
Buffer: 10 mM Tris-HCl, 200 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Apr 2
Nonstructural Proteins 7 and 8 of Feline Coronavirus Form a 2:1 Heterotrimer That Exhibits Primer-Independent RNA Polymerase Activity Journal of Virology 86(8):4444-4454 (2012)
Xiao Y, Ma Q, Restle T, Shang W, Svergun D, Ponnusamy R, Sczakiel G, Hilgenfeld R
RgGuinier 3.0 nm