Structure of ThiM from Vitamin B1 biosynthetic pathway of Staphylococcus aureus - Insights into a novel pro-drug approach addressing MRSA infections.

Drebes J, Künz M, Windshügel B, Kikhney AG, Müller IB, Eberle RJ, Oberthür D, Cang H, Svergun DI, Perbandt M, Betzel C, Wrenger C, Sci Rep 6:22871 (2016) Europe PMC

SASDAX8 – Ribokinase ThiM

Ribokinase ThiM
MWexperimental 77 kDa
MWexpected 89 kDa
VPorod 145 nm3
log I(s) 4.72×101 4.72×100 4.72×10-1 4.72×10-2
Ribokinase ThiM small angle scattering data  s, nm-1
ln I(s)
Ribokinase ThiM Guinier plot ln 4.73×101 Rg: 3.0 nm 0 (3.0 nm)-2 s2
(sRg)2I(s)/I(0)
Ribokinase ThiM Kratky plot 1.104 0 √3 sRg
p(r)
Ribokinase ThiM pair distance distribution function Rg: 2.9 nm 0 Dmax: 9 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Ribokinase ThiM CORAL model
log I(s)
 s, nm-1
Ribokinase ThiM DAMMIF model
Synchrotron SAXS data from solutions of Ribokinase ThiM in 50 mM Potassium phosphate 10 mM MgCl2, pH 7.5 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1.3 and 9.9 mg/ml were measured at 12°C. Eight successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Storage temperature = UNKNOWN

Tags: X33
Ribokinase ThiM (ThiM)
Mol. type   Protein
Organism   Staphylococcus aureus
Olig. state   Trimer
Mon. MW   29.8 kDa
Sequence   FASTA
 
PDB ID   5CM5