| MWI(0) | 197 | kDa |
| MWexpected | 215 | kDa |
| VPorod | 355 | nm3 |
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log I(s)
2.00×104
2.00×103
2.00×102
2.00×101
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s, nm-1
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Molecular flexibility of the condensin subunit Ycs4 is modulated by kleisin binding
Karen Manalastas-Cantos.SASDJ42 – Pentameric assembly of condensin complex subunits 1 and 2 (Ycs4-Brn1L-Brn1C) with subunits of the SMC hinge domain-containing protein (Smc4hd)
Condensin complex subunit 1Condensin complex subunit 2, 225-418
Condensin complex subunit 2, 776-898
SMC hinge domain-containing protein, 263-466
SMC hinge domain-containing protein, 1367-1542
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Fits and models
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Synchrotron SAXS data from solutions of the condensin Ycs4-Brn1L-Brn1C complex bound to subunits of Smc4hd in 25 mM Tris, 300 mM NaCl, 1mM DTT, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.50 mg/ml was measured at 20°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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