Structural and functional characterization of the Severe fever with thrombocytopenia syndrome virus L protein

Vogel D, Thorkelsson S, Quemin E, Meier K, Kouba T, Gogrefe N, Busch C, Reindl S, Günther S, Cusack S, Grünewald K, Rosenthal M, (2020) DOI

SASDHQ8 – Severe fever with thrombocytopenia syndrome virus (SFTSV) full-length L protein

Severe fever with thrombocytopenia syndrome virus L protein - RNA-dependent RNA polymerase
MWexperimental 242 kDa
MWexpected 238 kDa
VPorod 483 nm3
log I(s) 8.50×10-2 8.50×10-3 8.50×10-4 8.50×10-5
Severe fever with thrombocytopenia syndrome virus L protein - RNA-dependent RNA polymerase small angle scattering data  s, nm-1
ln I(s)
Severe fever with thrombocytopenia syndrome virus L protein - RNA-dependent RNA polymerase Guinier plot ln 8.50×10-2 Rg: 4.5 nm 0 (4.5 nm)-2 s2
(sRg)2I(s)/I(0)
Severe fever with thrombocytopenia syndrome virus L protein - RNA-dependent RNA polymerase Kratky plot 1.104 0 √3 sRg
p(r)
Severe fever with thrombocytopenia syndrome virus L protein - RNA-dependent RNA polymerase pair distance distribution function Rg: 4.4 nm 0 Dmax: 14.3 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of SFTSV full-length L protein in 50 mM HEPES(NaOH) pH 7.0, 500 mM NaCl, 5% (w/v) glycerol, 2 mM DTT, were collected on the EMBL P12 beam line at PETRA III (Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123987 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 25 μl sample at 7.4 mg/ml was injected at a 0.35 ml/min flow rate onto a GE Superdex 200 Increase 5/150 column at 20.2°C. 600 successive 0.995 second frames were collected through the entire SEC-elution. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted from data frames measured from the sample elution peak.

Severe fever with thrombocytopenia syndrome virus L protein - RNA-dependent RNA polymerase (SFTSV L protein)
Mol. type   Protein
Organism   SFTS virus AH12
Olig. state   Monomer
Mon. MW   238.1 kDa
 
UniProt   F1BV96 (1-2084)
Sequence   FASTA