| MWI(0) | 34 | kDa |
| MWexpected | 35 | kDa |
| VPorod | 50 | nm3 |
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log I(s)
4.69×101
4.69×100
4.69×10-1
4.69×10-2
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s, nm-1
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A salivary factor binds a cuticular protein and modulates biting by inducing morphological changes in the mosquito labrum.
Arnoldi I, Mancini G, Fumagalli M, Gastaldi D, D'Andrea L, Bandi C, Di Venere M, Iadarola P, Forneris F, Gabrieli P, Curr Biol (2022) Europe PMCSASDJX7 – Labrum-Interacting Proteins from Saliva 2 (34k-2) - SEC-SAXS
Similar to Aedes aegypti 34 kDa salivary secreted protein 34k-2|
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Fits and models
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Synchrotron SAXS data from solutions of labrum-interacting protein from saliva in 25 mM HEPES/NaOH, 0.1 M NaCl, pH 8 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Size exclusion chromatography coupled to SAXS (SEC-SAXS) was carried out at at 20°C using Nexera High Pressure Liquid Chromatography system (HPLC; Shimadzu). 50 μL of LIPS-2 concentrated at 10 mg/mL were injected into a Superdex 75 3.2/300 PC (GE Healthcare), pre-equilibrated with 25 mM HEPES/NaOH, 100 mM NaCl, pH 8.0. 82 successive 1 second frames were collected through the sample elution peak and processed using CHROMIXS that included the identification and subtraction of an appropriate solvent-blank.
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