The periplasmic chaperone Skp prevents misfolding of the secretory lipase A from Pseudomonas aeruginosa

Papadopoulos A, Busch M, Reiners J, Hachani E, Baeumers M, Berger J, Schmitt L, Jaeger K, Kovacic F, Smits S, Kedrov A, Frontiers in Molecular Biosciences 9 (2022) DOI

SASDNM5 – Skp-like protein, SKP

Skp-like protein
MWI(0) 55 kDa
MWexpected 55 kDa
VPorod 172 nm3
log I(s) 4.02×10-2 4.02×10-3 4.02×10-4 4.02×10-5
Skp-like protein small angle scattering data  s, nm-1
ln I(s)
Skp-like protein Guinier plot ln 4.03×10-2 Rg: 3.5 nm 0 (3.5 nm)-2 s2
(sRg)2I(s)/I(0)
Skp-like protein Kratky plot 1.104 0 √3 sRg
p(r)
Skp-like protein pair distance distribution function Rg: 3.5 nm 0 Dmax: 11.1 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Skp-like protein, SKP Rg histogram Rg, nm
Skp-like protein EOM/RANCH model
Skp-like protein EOM/RANCH model
Skp-like protein EOM/RANCH model
Skp-like protein EOM/RANCH model
log I(s)
 s, nm-1
Skp-like protein GASBOR model
SAXS data from solutions of SKP in 20 mM Tris, 100 mM NaCl, 10 % glycerol, pH 8 were collected using a Xenocs Xeuss 2.0 Q-Zoom instrument equipped with a Pilatus3 R 300 K detector and a GENIX 3D CU Ultra Low Divergence X-ray beam delivery system (Center for Structural Studies, Heinrich-Heine-University, Düsseldorf, Germany) at a sample-detector distance of 0.6 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.20 mg/ml was measured at 15°C. 24 successive 600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Skp-like protein (SKP)
Mol. type   Protein
Organism   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Olig. state   Trimer
Mon. MW   18.4 kDa
 
UniProt   Q9HXY5 (23-168)
Sequence   FASTA
 
ALPHAFOLD ID   Q9HXY5