| MWexperimental | 44 | kDa |
| MWexpected | 46 | kDa |
| VPorod | 54 | nm3 |
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log I(s)
1.15×10-2
1.15×10-3
1.15×10-4
1.15×10-5
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s, nm-1
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Structural flexibility of the tetanus neurotoxin revealed by crystallographic and solution scattering analyses.
Zhang CM, Imoto Y, Hikima T, Inoue T, J Struct Biol X 5:100045 (2021) Europe PMCSASDJN5 – Isolated translocation domain of tetanus nerotoxin (TeNT/iHn)
Tetanus toxin (C467S)|
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Fits and models
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Rg, nm
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Synchrotron SAXS data from solutions of the isolated translocation domain of the tetanus nerotoxin (TeNT/iHn) in 10 mM HEPES 100 mM NaCl, pH 7.4 were collected on the BL45XU beam line at SPring-8 (Hyōgo Prefecture, Japan) using a Pilatus3 X 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 5 mg/ml was injected at a 0.75 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 1200 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Storage temperature = UNKNOWN |
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