Cooperation between intrinsically disordered and ordered regions of Spt6 regulates nucleosome and Pol II CTD binding, and nucleosome assembly.

Kasiliauskaite A, Kubicek K, Klumpler T, Zanova M, Zapletal D, Koutna E, Novacek J, Stefl R, Nucleic Acids Res (2022) Europe PMC

SASDKZ9 – Transcription elongation factor SPT6 - ΔN Spt6

Transcription elongation factor SPT6 - ΔN Spt6 variant
MWexperimental 147 kDa
MWexpected 132 kDa
VPorod 288 nm3
log I(s) 6.87×103 6.87×102 6.87×101 6.87×100
Transcription elongation factor SPT6 - ΔN Spt6 variant small angle scattering data  s, nm-1
ln I(s)
Transcription elongation factor SPT6 - ΔN Spt6 variant Guinier plot ln 6.88×103 Rg: 4.7 nm 0 (4.7 nm)-2 s2
(sRg)2I(s)/I(0)
Transcription elongation factor SPT6 - ΔN Spt6 variant Kratky plot 1.104 0 √3 sRg
p(r)
Transcription elongation factor SPT6 - ΔN Spt6 variant pair distance distribution function Rg: 4.6 nm 0 Dmax: 14.4 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Transcription elongation factor SPT6 - ΔN Spt6 Rg histogram Rg, nm
log I(s)
 s, nm-1
Transcription elongation factor SPT6 - ΔN Spt6 variant CORAL model
Synchrotron SAXS data from solutions of Transcription elongation factor SPT6 - ΔN Spt6 in 25 mM Hepes; 150 NaCl; 0.5 mM EDTA; 5% glycerol; 1 mM DTT, pH 7.5 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.81 mg/ml was measured at 20°C. 20 successive 0.450 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Transcription elongation factor SPT6 - ΔN Spt6 variant (ΔN Spt6)
Mol. type   Protein
Organism   Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Olig. state   Monomer
Mon. MW   132.4 kDa
 
UniProt   P23615
Sequence   FASTA
 
PDB ID   3PSI