BusR senses bipartite DNA binding motifs by a unique molecular ruler architecture.

Bandera AM, Bartho J, Lammens K, Drexler DJ, Kleinschwärzer J, Hopfner KP, Witte G, Nucleic Acids Res (2021) Europe PMC

SASDK74 – Streptococcus agalactiae transcription factor BusR - RCK_C domain

Transcriptional repressor BusR RCK_C domain
MWexperimental 21 kDa
MWexpected 22 kDa
VPorod 44 nm3
log I(s) 1.46×10-2 1.46×10-3 1.46×10-4 1.46×10-5
Transcriptional repressor BusR RCK_C domain small angle scattering data  s, nm-1
ln I(s)
Transcriptional repressor BusR RCK_C domain Guinier plot ln 1.47×10-2 Rg: 1.9 nm 0 (1.9 nm)-2 s2
(sRg)2I(s)/I(0)
Transcriptional repressor BusR RCK_C domain Kratky plot 1.104 0 √3 sRg
p(r)
Transcriptional repressor BusR RCK_C domain pair distance distribution function Rg: 1.9 nm 0 Dmax: 6.4 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Transcriptional repressor BusR RCK_C domain OTHER model
Synchrotron SAXS data from solutions of the BusR - RCK_C domain in 100 mM NaCl, 30mM HEPES, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123981 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 8.70 mg/ml was measured at 10°C. 30 successive 0.095 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Transcriptional repressor BusR RCK_C domain (RCK_C)
Mol. type   Protein
Organism   Streptococcus agalactiae serotype III (strain NEM316)
Olig. state   Dimer
Mon. MW   11.0 kDa
 
UniProt   Q8E533 (134-213)
Sequence   FASTA
 
PDB ID   7B5W