Deckers B,
Vercauteren S,
De Kock V,
Martin C,
Lazar T,
Herpels P,
Dewachter L,
Verstraeten N,
Peeters E,
Ballet S,
Michiels J,
Galicia C,
Versées W,
Nucleic Acids Res
(2023)
Europe PMC
Synchrotron SAXS data from solutions of raw YbiB-ObgE GTPase complex in 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 2 mM DTT, 5% glycerol, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 7.6 mg/ml was injected at a 0.60 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 25°C. 47 successive 0.995 second frames were collected through the sample SEC-elution peak (from a total of 1798 data frames measured from the column eluate). The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
s, nm-1
