Synchrotron SAXS data from solutions of the vaccinia virus polymerase holoenzyme E9-A20-D4 in 200 mM NaCl, 20 mM Tris-HCl and 1 mM TCEP, pH 8.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 45.00 μl sample at 2 mg/ml was injected at a 0.10 ml/min flow rate onto a GE Superdex 200 Increase 3.2/300 column at 20°C. 2000 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The E9-A20-D4 polymerase holoenzyme with a 1:1:1 stoichiometry.
s, nm-1
