Structure of Vibrio collagenase VhaC provides insight into the mechanism of bacterial collagenolysis.

Wang Y, Wang P, Cao HY, Ding HT, Su HN, Liu SC, Liu G, Zhang X, Li CY, Peng M, Li F, Li S, Chen Y, Chen XL, Zhang YZ, Nat Commun 13(1):566 (2022) Europe PMC

SASDMU3 – Overall conformation and interdomain arrangement of Vibrio collagenase VhaC in solution.

Vibrio collagenase VhaC
MWexperimental 69 kDa
MWexpected 90 kDa
VPorod 148 nm3
log I(s) 1.56×101 1.56×100 1.56×10-1 1.56×10-2
Vibrio collagenase VhaC small angle scattering data  s, nm-1
ln I(s)
Vibrio collagenase VhaC Guinier plot ln 1.56×101 Rg: 4.3 nm 0 (4.3 nm)-2 s2
(sRg)2I(s)/I(0)
Vibrio collagenase VhaC Kratky plot 1.104 0 √3 sRg
p(r)
Vibrio collagenase VhaC pair distance distribution function Rg: 4.5 nm 0 Dmax: 17.6 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Vibrio collagenase VhaC CORAL model
log I(s)
 s, nm-1
Vibrio collagenase VhaC DAMMIN model
Synchrotron SAXS data from solutions Vibrio collagenase VhaC in 10 mM Tris-HCl, 100 mM NaCl, pH 8 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facility (SSRF; Shanghai, China) using a Pilatus 1M detector at a wavelength of λ = 0.0923 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 10°C. 20 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN

Vibrio collagenase VhaC (VhaC)
Mol. type   Protein
Organism   Vibrio harveyi
Olig. state   Monomer
Mon. MW   89.6 kDa
Sequence   FASTA