Cotranscriptional folding of the lncRNA Xist A-repeats indicates a modular structure

Alisha Jones.

SASDPM5 – SAXS curve of RNA Xist A-repeats (5 repeats)

Xist A-repeat lncRNA 5 repeats
MWexperimental 77 kDa
MWexpected 81 kDa
VPorod 186 nm3
log I(s) 2.05×10-1 2.05×10-2 2.05×10-3 2.05×10-4
Xist A-repeat lncRNA 5 repeats small angle scattering data  s, nm-1
ln I(s)
Xist A-repeat lncRNA 5 repeats Guinier plot ln 2.05×10-1 Rg: 7.7 nm 0 (7.7 nm)-2 s2
(sRg)2I(s)/I(0)
Xist A-repeat lncRNA 5 repeats Kratky plot 1.104 0 3 sRg
Dmax: 30 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of RNA Xist A-repeats (5 repeats) in 50 mM HEPES, 150 mM NaCl, 2 mM MgCl2, pH 7 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.48 mg/ml was measured at 25°C. One 0.145 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Xist A-repeat lncRNA 5 repeats (5 repeats)
Mol. type   RNA
Olig. state   Monomer
Mon. MW   81.2 kDa
Sequence   FASTA