Structural analyses of an RNA stability element interacting with poly(A)

Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J, Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021) DOI

SASDKE3 – double Element for Nuclear Expression (dENE) Crystal Structure + Poly(A)20

Xtal dENE + Poly(A)20
MWexperimental 35 kDa
MWexpected 34 kDa
log I(s) 1.97×100 1.97×10-1 1.97×10-2 1.97×10-3
Xtal dENE + Poly(A)20 small angle scattering data  s, nm-1
ln I(s)
Xtal dENE + Poly(A)20 Guinier plot ln 1.97×100 Rg: 3.2 nm 0 (3.2 nm)-2 s2
(sRg)2I(s)/I(0)
Xtal dENE + Poly(A)20 Kratky plot 1.104 0 √3 sRg
p(r)
Xtal dENE + Poly(A)20 pair distance distribution function Rg: 3.4 nm 0 Dmax: 11.8 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of double Element for Nuclear Expression (dENE) Crystal Structure + Poly(A)20 in 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH 7 were collected on the G1 beam line at the Cornell High Energy Synchrotron Source (CHESS) storage ring (Ithaca, NY, USA) using a Eiger X 1M detector at a sample-detector distance of 1.7 m and at a wavelength of λ = 0.12 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.70 mg/ml was measured at 22°C. 60 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Xtal dENE + Poly(A)20 (Xta dENE + Poly(A)20)
Mol. type   RNA
Olig. state   Monomer
Mon. MW   34.5 kDa
Sequence   FASTA