Hybrid Structural Analysis of the Arp2/3 Regulator Arpin Identifies Its Acidic Tail as a Primary Binding Epitope.

Fetics S, Thureau A, Campanacci V, Aumont-Nicaise M, Dang I, Gautreau A, Pérez J, Cherfils J, Structure 24(2):252-60 (2016) Europe PMC

SASDBX2 – Zebrafish Arpin in complex with the ankyrin repeat domains of human Tankyrase 2 (489-469)

Zebrafish arpin/human tankyrase 2 ankyrin repeat domain complex
MWexperimental 38 kDa
MWexpected 43 kDa
VPorod 58 nm3
log I(s) 7.20×10-2 7.20×10-3 7.20×10-4 7.20×10-5
Zebrafish arpin/human tankyrase 2 ankyrin repeat domain complex small angle scattering data  s, nm-1
ln I(s)
Zebrafish arpin/human tankyrase 2 ankyrin repeat domain complex Guinier plot ln 7.20×10-2 Rg: 3.3 nm 0 (3.3 nm)-2 s2
(sRg)2I(s)/I(0)
Zebrafish arpin/human tankyrase 2 ankyrin repeat domain complex Kratky plot 1.104 0 3 sRg
p(r)
Zebrafish arpin/human tankyrase 2 ankyrin repeat domain complex pair distance distribution function Rg: 3.4 nm 0 Dmax: 12.6 nm

Data validation


There are no models related to this curve.

X-ray synchrotron radiation scattering data from solutions of zebrafish arpin bound to the ankyrin repeat domains of human Tankyrase-2 (amino acids 489-649) in 50mM HEPES, pH 7.5, 100mM NaCl, 1mM TCEP were collected at 15 C on the SWING Beamline, Synchrotron Soleil, Gif Sur Yvette, France using a PCCD170170 Aviex detector. These data are derived from size-exclusion chromatography-SAXS. Note: The original SAXS data where the momentum transfer s in inverse Angstroms (s = 4π sin θ/λ, where 2θ is the scattering angle) is included in the zip file associated with this entry. Refer to UniProt ID Q9H2K2 (amino acids 489-649) and Q1LWJ6 for additional sequence information.

Due to high concentration at the elution peak, the HPLC UV detector was saturated and the concentrations of the sample was consequently underestimated. In this case, the molecular mass is overestimated when assessing this parameter from I(0) and concentration.

Zebrafish arpin/human tankyrase 2 ankyrin repeat domain complex (Arpin/tankyrase)
Mol. type   Protein
Organism   Danio rerio / Homo sapiens
Olig. state   Monomers
Mon. MW   43.0 kDa
Sequence   FASTA