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Synchrotron SAXS data from solutions of TRIM28 RBCC_R184D in complex with MBP-Krab ZFP809 in 10 mM Tris 300 mM NaCl 0.1 mM TCEP, pH 8 were collected using size-exclusion chromatography SAXS (SEC-SAXS) on the SAXS/WAXS beam line at the Australian Synchrotron (Melbourne, Australia) using a Pilatus 1M detector at a sample-detector distance of 3.3 m and at a wavelength of λ = 0.113 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 16 °C using the GE-Healthcare S200 5/150 analytical SEC column at a flow rate of 0.5 mL/min. Two successive 50 μL samples were loaded at 1:1 RBCC to MBP-Krab and 1:1.5 RBCC to MBP-Krab molar ratios, at concentrations of 11.2 and 14.3 mg/mL, respectively. The data were collected through the SEC peaks of each individual run as a series of 48 x 1 second exposures . The data were normalized to the intensity of the transmitted beam and radially averaged and the the scattering of an appropriate solvent-blank was subtracted. Both samples yielded identical scattering curves after buffer subtraction and therefore have been scaled and merged to generate the final SAXS profile displayed in this entry.
Storage temperature = UNKNOWN. SEC column = UNKNOWN. Sample injection volume = UNKNOWN. Flow rate = UNKNOWN
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s, nm-1
